{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T23:51:40Z","timestamp":1784073100418,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T00:00:00Z","timestamp":1567123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011002","name":"National Natural Science Foundation of China-Yunnan Joint Fund","doi-asserted-by":"publisher","award":["81271838"],"award-info":[{"award-number":["81271838"]}],"id":[{"id":"10.13039\/501100011002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background &amp; Aims<\/jats:title><jats:p>Hepatitis B virus (HBV) infection is the most critical factor underlying liver cirrhosis and hepatocellular carcinoma worldwide. IL\u20101\u03b2 and IL\u201018, generated by activation of the inflammasome\/caspase\u20101 signaling pathway, play important roles in the control and clearance of HBV. However, the specific relationship between the inflammasome response and IFN\u2010\u03b1 resistance or viral persistence is yet to be established.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood samples of patients and supernatant fractions of HBV cell lines were collected for analysis and the effects on inflammasome activation and IL\u20101\u03b2 production evaluated via enzyme\u2010linked immunosorbent assay (ELISA), western blot, quantitative RT\u2010PCR and immunofluorescence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IL\u20101\u03b2 and IL\u201018 levels produced in sera of IFN\u2010\u03b1 non\u2010responders were significantly lower than those of responders and normal donors. Additionally, expression of IL\u20101\u03b2 and inflammasome components was decreased in peripheral blood mononuclear cells (PBMC) of non\u2010responders, compared with those of responders. In vitro experiments on HepG2, HepG2.2.15 and HepAD38 cell lines showed that HBV induces a significant decrease in IL\u20101\u03b2 production through inhibiting activation of the NF\u2010\u03baB signaling and inflammasome\/caspase\u20101 pathways. And hepatitis B virus polymerase (HBV\u2010Pol) appeared crucial for these inhibitory effects of HBV.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>IL\u20101\u03b2 production is suppressed in HBV carriers and IFN\u2010\u03b1 non\u2010responders. HBV induces a significant decrease in IL\u20101\u03b2 production through inhibiting the NF\u2010\u03baB signaling and inflammasome pathways, for which HBV\u2010Pol is a crucial requirement.<\/jats:p><jats:p><jats:bold>Trial approval number:<\/jats:bold> 20\u00a0173\u00a0402.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.14214","type":"journal-article","created":{"date-parts":[[2019,8,16]],"date-time":"2019-08-16T18:02:58Z","timestamp":1565978578000},"page":"2273-2284","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["HBV\u2010Pol is crucial for HBV\u2010mediated inhibition of inflammasome activation and IL\u20101\u03b2 production"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1779-4807","authenticated-orcid":false,"given":"Qingsong","family":"Lei","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases Chongqing Key Laboratory of Infectious Diseases and 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