{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T10:58:05Z","timestamp":1790247485846,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T00:00:00Z","timestamp":1568073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background &amp; Aims<\/jats:title><jats:p>The eradication of Hepatitis C (HCV) infection by direct\u2010acting antiviral (DAAs) agents has been linked to an amelioration of liver synthesis and regression of fibrosis. Although changes in number and type of circulating microvesicles (MVs) have been reported in cirrhosis, conclusive data on the effect of DAAs treatment on MVs profile in HCV cirrhotic patients remain scarce.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We measured the levels of endothelial, platelet and hepatocyte MVs, as well as MVs\u2010expressing versican core protein (VCAN+) in patients with HCV\u2010related cirrhosis at baseline, end of treatment (EOT), at 12, 24 and 48\u00a0weeks (W) after EOT by new generation flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifty\u2010eight patients were enrolled (86% Child's A). MVs were increased at EOT vs baseline, though only platelet MVs revealed a statistically significant difference (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01). MV levels did not change significantly after EOT notwithstanding a steady downward trend towards baseline levels. Conversely, VCAN\u00a0+\u00a0MVs dropped significantly at EOT (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001) and remained low throughout the follow\u2010up. Hepatocyte MVs significantly correlated with liver stiffness (r\u00a0=\u00a0.40, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.0021). Eight composite outcomes occurred during the 1\u2010year follow\u2010up: three portal vein thromboses (PVTs), two hepatocellular carcinomas (HCCs) and three liver decompensation. Child's B, the presence of F2 oesophageal varices (OR for interaction 19.2 [95% CI 1.45\u2010253.7], <jats:italic>P<\/jats:italic>\u00a0=\u00a0.023) and platelet MVs (OR 1.026 [95% CI 1.00\u20101.05, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.023) correlated significantly with clinical outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>VCAN\u00a0+\u00a0MVs appear to mirror the profibrotic status of the cirrhotic disease; hepatocyte MVs correlate with liver stiffness and increased platelet MV levels could be associated with a worse clinical outcome.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.14234","type":"journal-article","created":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T18:30:51Z","timestamp":1566930651000},"page":"913-920","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Changes in plasma circulating microvesicles in patients with HCV\u2010related cirrhosis after treatment with direct\u2010acting antivirals"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0377-3741","authenticated-orcid":false,"given":"Elena","family":"Campello","sequence":"first","affiliation":[{"name":"Department of Medicine Thrombotic and Haemorrhagic Diseases Unit Padova University Hospital Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia M.","family":"Radu","sequence":"additional","affiliation":[{"name":"Department of Medicine Thrombotic and Haemorrhagic Diseases Unit Padova University Hospital Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6734-7178","authenticated-orcid":false,"given":"Alberto","family":"Zanetto","sequence":"additional","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology Gastroenterology and Multivisceral Transplant Unit Padova University Hospital Padova 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