{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:16:04Z","timestamp":1787318164544,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T00:00:00Z","timestamp":1577664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700748"],"award-info":[{"award-number":["81700748"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background &amp; Aims<\/jats:title>\n                    <jats:p>Nonalcoholic fatty liver disease encompasses isolated steatosis or nonalcoholic fatty liver and nonalcoholic steatohepatitis (NASH). NASH develops from isolated steatosis with obscure driving forces. We aim to identify key factors promoting this transition.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Following 21\u2010week of high\u2010fat diet feeding, obese mice were classified into two groups termed as isolated steatosis and NASH based on hematoxylin\u2010eosin staining of liver histology. The integrated multi\u2010omics analysis of lipidome, transcriptome and gut microbiome were performed in mice with isolated steatosis and NASH, and confirmed in human samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Livers in mice with NASH lost most lipids, and the transcriptional landscape was also changed dramatically in mice with NASH in relative to mice with isolated steatosis. Plasma lipidome analysis demonstrated a very clear difference between these two groups of mice, which was partially recapitulated in serum of patients with isolated steatosis and NASH. The microbiota composition revealed that\n                      <jats:italic>Bacteroides<\/jats:italic>\n                      genus and\n                      <jats:italic>Bacteroides uniformis<\/jats:italic>\n                      species decreased while\n                      <jats:italic>Mucispirillum<\/jats:italic>\n                      genus and\n                      <jats:italic>Mucispirillum schaedleri<\/jats:italic>\n                      species increased largely in mice with NASH. More importantly, we found that\n                      <jats:italic>Bacteroides uniformis<\/jats:italic>\n                      correlated positively with triglycerides (TGs) and negatively with free fatty acids (FFAs) and PE(18:1\/20:4), while\n                      <jats:italic>Mucispirillum schaedleri<\/jats:italic>\n                      correlated positively with FFAs, LysoPC(20:3), LysoPC(20:4) and DG(16:1\/18:2). Mechanically, administration of\n                      <jats:italic>Bacteroides uniformis<\/jats:italic>\n                      increased specific TGs, and decreased hepatic injury and inflammation in diet\u2010induced mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Overall, through multi\u2010omics integration, we identified a microbiota\u2010lipid axis promoting the initiation of NASH from isolated steatosis, which might provide a novel perspective on NASH pathogenesis and treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/liv.14316","type":"journal-article","created":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T07:00:30Z","timestamp":1575010830000},"page":"622-637","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Coordinated changes of gut microbiome and lipidome differentiates nonalcoholic steatohepatitis (NASH) from isolated steatosis"],"prefix":"10.1111","volume":"40","author":[{"given":"Minyi","family":"Qian","sequence":"first","affiliation":[{"name":"School of Basic Medicine and Clinical Pharmacy State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyang","family":"Hu","sequence":"additional","affiliation":[{"name":"School of life Science and Technology China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Nanjing University of Chinese Medicine Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danyang","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Basic Medicine and Clinical Pharmacy State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilei","family":"Wang","sequence":"additional","affiliation":[{"name":"Clinical Metabolomics Center China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuyue","family":"Pan","sequence":"additional","affiliation":[{"name":"School of Basic Medicine and Clinical Pharmacy State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xubin","family":"Duan","sequence":"additional","affiliation":[{"name":"School of Basic Medicine and Clinical Pharmacy State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingsheng","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Basic Medicine and Clinical 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