{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T20:26:18Z","timestamp":1759177578108,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T00:00:00Z","timestamp":1596067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background &amp; Aims<\/jats:title><jats:p>The presence of baseline resistance\u2010associated substitutions (RASs) reduced sustained virologic response (SVR) rates in chronic hepatitis C virus (HCV) genotype 1a infected patients treated with Elbasvir\/Grazoprevir (EBR\/GZR). This study aimed to evaluate the frequency of NS5A RASs and treatment outcomes in patients for whom EBR\/GZR was intended.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We sequenced NS5A in 832 samples from German genotype1a\u2010infected DAA\u2010na\u00efve patients population\u2010based, which were collected in the European Resistance Database. Treatment outcomes and clinical parameters were evaluated in 519 of such patients retrospectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, 6.5% of patients harboured EBR\u2010specific NS5A RASs at baseline, including Q30H\/R (3.3%), L31M (1.8%), Y93H (1.6%) and other individual variants. Antiviral treatment, including EBR\/GZR, was initiated in 88% of the patients. In the absence of RASs, the majority of patients received EBR\/GZR for 12\u00a0weeks (57%) and the SVR rate was 97% compared to 99% SVR achieved using other DAA regimens (LDV\/SOF\u00a0\u00b1\u00a0RBV, G\/P, PrOD\u00a0+\u00a0RBV, VEL\/SOF). Various regimens were used in the presence of RASs and SVR rates were high following treatment with LDV\/SOF (100%), G\/P (83%), PrOD\/RBV (100%), VEL\/SOF (100%), SMV\/SOF (100%) and EBR\/GZR\u00a0+\u00a0RBV for 16\u00a0weeks (100%). However, two patients received EBR\/GZR for 16\u00a0weeks without RBV and one relapsed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>EBR\/GZR treatment with or without RBV for 12 or 16\u00a0weeks according to a baseline RAS analysis was highly effective with \u226597% SVR in patients with genotype 1a. EBR\/GZR without RBV should be avoided in patients with RASs. High SVR rates were also achieved using other 8\u2010 or 12\u2010week DAA regimens.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.14591","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T19:23:55Z","timestamp":1594236235000},"page":"2660-2671","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Treatment outcomes in hepatitis C virus genotype 1a infected patients with and without baseline NS5A resistance\u2010associated substitutions"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6990-3257","authenticated-orcid":false,"given":"Julia","family":"Dietz","sequence":"first","affiliation":[{"name":"Department of Internal Medicine 1 University Hospital Goethe University Frankfurt Germany"},{"name":"German Center for Infection Research (DZIF) External Partner Site Frankfurt Germany"},{"name":"Johann Wolfgang Goethe-Universit\u00e4t Frankfurt am Main Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes","family":"Vermehren","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine 1 University Hospital Goethe University Frankfurt Germany"},{"name":"German Center for Infection Research (DZIF) External Partner Site Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrin","family":"Matschenz","sequence":"additional","affiliation":[{"name":"Institute for Interdisciplinary Medicine IFI Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Buggisch","sequence":"additional","affiliation":[{"name":"Institute for Interdisciplinary Medicine IFI Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hartwig","family":"Klinker","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Division of Infectious Diseases University Hospital W\u00fcrzburg W\u00fcrzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian","family":"Schulze zur Wiesch","sequence":"additional","affiliation":[{"name":"I. Department of Medicine University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"},{"name":"German Center for Infection Research (DZIF) Partner Site Hamburg\u2010L\u00fcbeck\u2010Borstel\u2010Riems Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holger","family":"Hinrichsen","sequence":"additional","affiliation":[{"name":"Gastroenterologisch\u2010Hepatologisches Zentrum Kiel Kiel Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai\u2010Henrik","family":"Peiffer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine 1 University Hospital Goethe University Frankfurt Germany"},{"name":"German Center for Infection Research (DZIF) External Partner Site Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiana","family":"Graf","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine 1 University Hospital Goethe University Frankfurt Germany"},{"name":"German Center for Infection Research (DZIF) External Partner Site Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Discher","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Section of Infectious Diseases Justus\u2010Liebig\u2010University Giessen Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janina","family":"Trauth","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Section of Infectious Diseases Justus\u2010Liebig\u2010University Giessen Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4224-4703","authenticated-orcid":false,"given":"J\u00f6rn M.","family":"Schattenberg","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I University Medical Center of the Johannes Gutenberg\u2010University Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2781-5088","authenticated-orcid":false,"given":"Felix","family":"Piecha","sequence":"additional","affiliation":[{"name":"I. 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