{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T10:45:09Z","timestamp":1790851509470,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T00:00:00Z","timestamp":1602720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DA1063\/3\u20102"],"award-info":[{"award-number":["DA1063\/3\u20102"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB 841 A5,A8"],"award-info":[{"award-number":["SFB 841 A5,A8"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Backgrounds &amp; Aims<\/jats:title><jats:p>As a result of the limited availability of in vivo models for hepatitis D virus (HDV), treatment options for HDV chronically infected patients are still scant. The discovery of sodium taurocholate cotransporting polypeptide (NTCP) as HDV entry receptor has enabled the development of new infection models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To comparatively assess the efficacy and persistence of HDV mono\u2010infection in murine and human hepatocytes in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice with humanized NTCP (hNTCP<jats:sup>ed84\u201087<\/jats:sup> mice) were generated by editing amino acid residues 84\u201087 of murine NTCP in C57BL\/6J mice. HDV infection was assessed in hNTCP<jats:sup>ed84\u201087<\/jats:sup> mice and in immune deficient uPA\/SCID\/beige (USB) mice, whose livers were reconstituted with human or murine (hNTCP<jats:sup>ed84\u201087<\/jats:sup>) hepatocytes. Livers were analysed between 5 and 42\u00a0days post\u2010HDV inoculation by qRT\u2010PCR, immunofluorescence and RNA in situ hybridization (ISH).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>hNTCP<jats:sup>ed84\u201087<\/jats:sup> mice could be infected with HDV genotype 1 or 3. ISH analysis demonstrated the presence of antigenomic HDV RNA positive murine hepatocytes with both genotypes, proving initiation of HDV replication. Strikingly, murine hepatocytes cleared HDV within 21\u00a0days both in immunocompetent hNTCP<jats:sup>ed84\u201087<\/jats:sup> mice and in immunodeficient USB mice xenografted with murine hepatocytes. In contrast, HDV infection remained stable for at least 42\u00a0days in human hepatocytes. Intrinsic innate responses were not enhanced in any of the HDV mono\u2010infected cells and livers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings suggest that in addition to NTCP, further species\u2010specific factors limit HDV infection efficacy and persistence in murine hepatocytes. Identifying such species barriers may be crucial to develop novel potential therapeutic targets of HDV.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.14677","type":"journal-article","created":{"date-parts":[[2020,9,30]],"date-time":"2020-09-30T19:49:34Z","timestamp":1601495374000},"page":"410-419","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Murine hepatocytes do not support persistence of Hepatitis D virus mono\u2010infection in vivo"],"prefix":"10.1111","volume":"41","author":[{"given":"Katja","family":"Giersch","sequence":"first","affiliation":[{"name":"I. Department of Internal Medicine University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennart","family":"Hermanussen","sequence":"additional","affiliation":[{"name":"I. Department of Internal Medicine University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tassilo","family":"Volz","sequence":"additional","affiliation":[{"name":"I. Department of Internal Medicine University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janine","family":"Kah","sequence":"additional","affiliation":[{"name":"I. Department of Internal Medicine University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena","family":"Allweiss","sequence":"additional","affiliation":[{"name":"I. 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