{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T20:44:03Z","timestamp":1775767443387,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T00:00:00Z","timestamp":1639094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF15OC0016692"],"award-info":[{"award-number":["NNF15OC0016692"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background &amp; Aims<\/jats:title><jats:p>Binge drinking is associated with an increased risk of liver disease. Morbidity and mortality of alcohol\u2010related liver disease (ALD) is associated with collagen deposition in the hepatic extracellular matrix (ECM). However, the acute effects of binge drinking on ECM turnover are unknown. We aimed to investigate the effects on hepatic ECM turnover following a binge drinking episode.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed a pathophysiological intervention study with 15 non\u2010alcoholic fatty liver disease (NAFLD) patients, 15 ALD patients and 10 healthy controls. We used 40% ethanol in 9\u00a0mg\/mL NaCl administered through a nasogastric tube to simulate binge drinking. Hepatic vein catheterisation allowed simultaneous hepatic\u2010 and systemic vein sampling. Markers of ECM formation and degradation were measured with competitive ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The interstitial matrix formation marker PRO\u2010C3 increased by 1.2\u00a0ng\/mL (10%, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001) 24\u00a0hours after binge drinking. In participants with existing liver fibrosis determined by elevated baseline PRO\u2010C3, hepatic levels increased by 0.09\u00a0ng\/mL (95% CI: 0.03\u20100.15, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.005) while systemic PRO\u2010C3 decreased 0.11\u00a0ng\/mL (95% CI: \u22120.15 to \u22120.06, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001) in 3\u00a0hours. PRO\u2010C8 increased by 30% (+0.9\u00a0ng\/mL, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.014) in liver\u2010diseased patients with F0\u2010F1 but not in any other group. Twenty\u2010four\u2010hour changes in systemic C3M and PRO\u2010C3 were not associated (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.911).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Binge drinking induced an acute burst of PRO\u2010C3 in healthy individuals and patients with liver disease. Markers of ECM degradation were not correlated to markers of ECM formation, suggesting that even a single episode of binge drinking promotes excessive hepatic fibrogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.15120","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T16:38:07Z","timestamp":1638290287000},"page":"92-101","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Binge drinking induces an acute burst of markers of hepatic fibrogenesis (PRO\u2010C3)"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4378-6256","authenticated-orcid":false,"given":"Nikolaj","family":"Torp","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology Odense University Hospital Odense Denmark"},{"name":"Institute of Clinical Research University of Southern Denmark Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mads","family":"Israelsen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Odense University Hospital Odense Denmark"},{"name":"Institute of Clinical Research University of Southern Denmark Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mette J.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Nordic Bioscience A\/S Herlev Hovedgade Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus P.","family":"\u00c5strand","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Odense University Hospital Odense Denmark"},{"name":"Institute of Clinical Research University of Southern Denmark Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pernille","family":"Juhl","sequence":"additional","affiliation":[{"name":"Nordic Bioscience A\/S Herlev Hovedgade Herlev Denmark"},{"name":"Department of Biomedical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stine","family":"Johansen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Odense University Hospital Odense Denmark"},{"name":"Institute of Clinical Research University of Southern Denmark Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camilla D.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Odense University Hospital Odense Denmark"},{"name":"Institute of Clinical Research University of Southern Denmark Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2846-3598","authenticated-orcid":false,"given":"Bj\u00f8rn","family":"Madsen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Odense University Hospital Odense Denmark"},{"name":"Institute of Clinical Research University of Southern Denmark Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7921-9707","authenticated-orcid":false,"given":"Ida F.","family":"Villesen","sequence":"additional","affiliation":[{"name":"Nordic Bioscience A\/S Herlev Hovedgade Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4256-140X","authenticated-orcid":false,"given":"Diana J.","family":"Leeming","sequence":"additional","affiliation":[{"name":"Nordic Bioscience A\/S Herlev Hovedgade Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1854-1924","authenticated-orcid":false,"given":"Maja","family":"Thiele","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Odense University Hospital Odense Denmark"},{"name":"Institute of Clinical Research University of Southern Denmark Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torben","family":"Hansen","sequence":"additional","affiliation":[{"name":"Novo Nordisk Foundation Center for Basic Metabolic Research University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5026-8740","authenticated-orcid":false,"given":"Morten","family":"Karsdal","sequence":"additional","affiliation":[{"name":"Nordic Bioscience A\/S Herlev Hovedgade Herlev Denmark"},{"name":"Department of Molecular Medicine Faculty of Health Sciences University of Southern Denmark Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksander","family":"Krag","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Odense University Hospital Odense Denmark"},{"name":"Institute of Clinical Research University of Southern Denmark Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,10]]},"reference":[{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1715733"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines7040099"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.13408"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.14770"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amepre.2017.12.021"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.27368"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.16111"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28948"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.30969"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2018.04.006"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2938-9"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2007.145573"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.10.041"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20701"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.30163"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.16513"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhepr.2021.100325"},{"issue":"3","key":"e_1_2_10_20_1","first-page":"303","article-title":"The neo\u2010epitope specific PRO\u2010C3 ELISA measures true formation of type III collagen associated with liver and muscle parameters","volume":"5","author":"Nielsen MJ","year":"2013","journal-title":"Am J Transl Res"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1755-1536-4-22"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.12820"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.14390"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108544"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.14806"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-30457-y"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep4.1135"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.16567"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123311"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2019.05.020"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2007.02.003"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28860"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23567"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008209009152157"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2016.56"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2016.05.023"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.14070"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2011.00946.x"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2010.03.012"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00476"}],"container-title":["Liver International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/liv.15120","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/liv.15120","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/liv.15120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T20:23:44Z","timestamp":1692822224000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/liv.15120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,10]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1111\/liv.15120"],"URL":"https:\/\/doi.org\/10.1111\/liv.15120","archive":["Portico"],"relation":{},"ISSN":["1478-3223","1478-3231"],"issn-type":[{"value":"1478-3223","type":"print"},{"value":"1478-3231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,10]]},"assertion":[{"value":"2021-05-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}