{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:57:10Z","timestamp":1790758630285,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T00:00:00Z","timestamp":1649721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005930","name":"ASCRS Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005930","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>Liver fibrosis results from a prolonged wound healing response to continued injury with excessive production of extracellular proteins. In patients with chronic liver disease, the monitoring of liver fibrosis dynamics is of high interest. Whilst markers of fibrogenesis exist, markers of hepatic fibrosis resolution remain an unmet clinical need. Thus, we sought to develop an assay quantifying a circulating proteolytic fragment of cross\u2010linked type <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> collagen as a biomarker of fibrolysis, testing its utility in two clinical cohorts of liver fibrosis of distinct aetiology and regressing endotype<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used a monoclonal antibody targeting the C\u2010telopeptide of type III collagen following C\u2010proteinase cleavage to develop and validate a neo\u2010epitope\u2010specific enzyme\u2010linked immunosorbent assay (CTX\u2010III). A potential fibrosis resolution marker, CTX\u2010III, was measured in two clinical cohorts of patients with obesity\u2010associated non\u2010alcoholic fatty liver disease undergoing bariatric surgery or hepatitis C virus infection from a clinical trial study evaluating the anti\u2010fibrotic effect of farglitazar.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CTX\u2010III was robust and specific for the targeted neo\u2010epitope with good reproducibility in EDTA plasma. We assessed type III collagen remodelling using a panel of biomarkers, including a type III collagen formation marker (PRO\u2010C3), degradation (C3M), and CTX\u2010III (fibrolysis). Net fibrolysis was increased in patients with non\u2010alcoholic fatty liver disease following bariatric surgery (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.001). Moreover, net fibrolysis identified spontaneous fibrotic regressors from stable and progressors (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05 and <jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.001) among hepatitis C virus infection patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Circulating CTX\u2010III as a marker of fibrolysis indicates the biomarker's beneficial use in assessing hepatic fibrosis resolution.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.15270","type":"journal-article","created":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T09:52:01Z","timestamp":1649152321000},"page":"1605-1617","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["An <scp>MMP<\/scp>\u2010degraded and cross\u2010linked fragment of type <scp>III<\/scp> collagen as a non\u2010invasive biomarker of hepatic fibrosis resolution"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4787-5563","authenticated-orcid":false,"given":"Martin","family":"Pehrsson","sequence":"first","affiliation":[{"name":"Faculty of Health and Medical Sciences, Department of Biomedical Sciences University of Copenhagen  Copenhagen Denmark"},{"name":"Biomarkers and Research Nordic Bioscience A\/S  Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina","family":"Manon\u2010Jensen","sequence":"additional","affiliation":[{"name":"Biomarkers and Research Nordic Bioscience A\/S  Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Sun","sequence":"additional","affiliation":[{"name":"Biomarkers and Research Nordic Bioscience A\/S  Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7921-9707","authenticated-orcid":false,"given":"Ida 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