{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:13:58Z","timestamp":1787015638037,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T00:00:00Z","timestamp":1676505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009781","name":"Australian Centre for HIV and Hepatitis Virology Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004752","name":"State Government of Victoria","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004752","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Alanine aminotransferase (ALT) measurement is essential for evaluation of liver disease. We validated a novel rapid point\u2010of\u2010care (POC) test for ALT1 against laboratory ALT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Stored plasma samples from adults with chronic liver disease (Test cohort\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0240; Validation cohort\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0491) were analysed using the BioPoint\u00ae antigen immunoassay POC ALT1 lateral flow test, which provides quantitative ALT results (Axxin handheld reader) or semi\u2010quantitative results (visual read, cut off 40\u2009IU\/ml). The accuracy of POC ALT1 to detect ALT\u2009&gt;\u200940\u2009IU\/L was determined by ROC analysis. In patients with chronic hepatitis B, treatment eligibility (EASL criteria) was determined using POC ALT1 and compared to laboratory ALT.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>POC ALT1 test had good accuracy for laboratory ALT\u2009&gt;\u200940\u2009IU\/L: AUROC 0.93 (95% CI: 0.89\u20130.96) in the Test cohort and AUROC 0.92 (95% CI: 0.88\u20130.95) in the Validation cohort. POC ALT1 cut off of 0.8 for ALT\u2009&gt;\u200940\u2009IU\/L maximised sensitivity (97%) and specificity (71%) in the Test cohort (42% laboratory ALT\u2009&gt;\u200940\u2009IU\/L) and yielded PPV 84% and NPV 91% in the Validation cohort (19% laboratory ALT\u2009&gt;\u200940\u2009IU\/L). Semi\u2010quantitative POC ALT1 had good accuracy for laboratory ALT in the Validation cohort (AUROC 0.85, 95% CI: 0.81\u20130.99; sensitivity 77% and specificity 93%). Combined with HBV DNA and transient elastography, both quantitative and semi\u2010quantitative POC ALT1 tests had good accuracy for excluding hepatitis B treatment needs (sensitivity 96%, specificity 78% and NPV 99%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The POC ALT1 test had good accuracy for elevated ALT levels and for determining treatment eligibility among people with chronic hepatitis B.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/liv.15531","type":"journal-article","created":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T07:33:26Z","timestamp":1675150406000},"page":"989-999","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Validation of a novel point\u2010of\u2010care test for alanine aminotransferase measurement: A pilot cohort study"],"prefix":"10.1111","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9269-6249","authenticated-orcid":false,"given":"Jessica","family":"Howell","sequence":"first","affiliation":[{"name":"Burnet Institute  Melbourne Australia"},{"name":"St Vincent's Hospital and University of Melbourne  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huy","family":"Van","sequence":"additional","affiliation":[{"name":"Burnet Institute  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minh D.","family":"Pham","sequence":"additional","affiliation":[{"name":"Burnet Institute  Melbourne Australia"},{"name":"School of Public Health and Preventive Medicine, Monash University  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohit","family":"Sawhney","sequence":"additional","affiliation":[{"name":"St Vincent's Hospital and University of Melbourne  Melbourne 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