{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:05:56Z","timestamp":1791515156892,"version":"4.3.4"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T00:00:00Z","timestamp":1699228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82070298"],"award-info":[{"award-number":["82070298"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170589"],"award-info":[{"award-number":["82170589"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>Alcohol consumption is a well\u2010established risk factor for the onset and progression of hepatic steatosis. Perilipin 5 (Plin5), a lipid droplet protein, is an important protective factor against hepatic lipotoxicity induced by excessive lipolysis, but its role and molecular mechanism in alcoholic liver disease (ALD) are not fully elucidated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The optimized National Institute on Alcohol Abuse and Alcoholism model was used to construct ALD model mice. Automatic biochemical analyser was used for Biochemical Parameters. The primary hepatocytes and Plin5\u2010overexpressed HepG2 cells (including full\u2010length Plin5 and Plin5 deleting 444\u2013464 aa) were used for in\u00a0vitro experiment. Haematoxylin and Eosin staining, Oil Red O staining, Bodipy 493\/503 staining, Periodic Acid\u2010Schiff staining, immunohistochemistry and JC\u20101 staining were used to evaluate cell morphology, lipids, glycogen, inflammation and membrane potential. Commercially kits are used to detect glycolipid metabolites, such as triglycerides, glycogen, glucose, reactive oxygen species, lactic acids, ketone bodies. Fluorescently labelled deoxyglucose, NBDG, was used for glucose intake. An XF96 extracellular flux analyser was used to determinate oxygen consumption rate in hepatocytes. The morphological and structural damage of mitochondria was evaluated by electron microscopy. Classical ultracentrifugation is used to separate the subcellular organelles of tissues and cells. Immunoblotting and qPCR were used to detect changes in mRNA and protein levels of related genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results showed that the expression of Plin5 in mouse livers was enhanced by alcohol intake, and Plin5 deficiency aggravated the alcohol\u2010induced liver injury. To clarify the mechanism, we found that Plin5 deficiency significantly elevated the hepatic NADH levels and ketone body production in the alcohol\u2010treated mice. As NADH elevation could promote the reduction of pyruvate into lactate and then inhibit the gluconeogenesis, alcohol\u2010treated Plin5\u2010deficient mice exhibited more lactate production and severer hypoglycemia. These results implied that Plin5 deficiency impaired the mitochondrial oxidative functions in the presence of alcohol. In addition, we demonstrated that Plin5 could be recruited onto mitochondria by alcohol, while Plin5 without mitochondrial targeting sequences lost its mitochondrial protection functions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Collectively, this study demonstrated that the mitochondrial Plin5 could protect the alcohol\u2010induced mitochondrial injury, which provides an important new insight on the roles of Plin5 in highly oxidative tissues.<\/jats:p><\/jats:sec>","DOI":"10.1111\/liv.15775","type":"journal-article","created":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T06:20:30Z","timestamp":1699338030000},"page":"357-369","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Perilipin 5 protects the mitochondrial oxidative functions and improves the alcoholic liver injury in mice"],"prefix":"10.1111","volume":"44","author":[{"given":"Xing","family":"Gao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital and School of Basic Medicine Fourth Military Medical University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lele","family":"Jian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cancer Biology, School of Basic Medicine Fourth Military Medical University  Xi'an Shaanxi China"},{"name":"Shaanxi Provincial Corps Chinese People's Armed Police Force  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Diagnosis, Tangdu Hospital Fourth Military Medical University  Xi'an Shaanxi 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