{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T06:12:27Z","timestamp":1783059147038,"version":"3.54.6"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:00:00Z","timestamp":1712880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:00:00Z","timestamp":1712880000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004480","name":"Natural Science Foundation of Shanxi Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004480","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Purpose<\/jats:title>\n                    <jats:p>Liver fibrosis is a wound\u2010healing reaction which is the main cause of chronic liver diseases worldwide. The activated hepatic stellate cell (aHSC) is the main driving factor in the development of liver fibrosis. Inhibiting autophagy of aHSC can prevent the progression of liver fibrosis, but inhibiting autophagy of other liver cells has opposite effects. Hence, targeted inhibition of autophagy in aHSC is quite necessary for the treatment of liver fibrosis, which prompts us to explore the targeted delivery system of small molecule autophagy inhibitor hydroxychloroquine (HCQ) that can target aHSC and alleviate the liver fibrosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The delivery system of HCQ@retinol\u2010liposome nanoparticles (HCQ@ROL\u2010LNPs) targeting aHSC was constructed by the film dispersion and pH\u2010gradient method. TGF\u2010\u03b2\u2010induced HSC activation and thioacetamide (TAA)\u2010induced liver fibrosis mice model were established, and the targeting ability and therapeutic effect of HCQ@ROL\u2010LNPs in liver fibrosis were studied subsequently in\u00a0vitro and in\u00a0vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>HCQ@ROL\u2010LNPs have good homogeneity and stability. They inhibited the autophagy of aHSC selectively by HCQ and reduced the deposition of extracellular matrix (ECM) and the damage to other liver cells. Compared with the free HCQ and HCQ@LNPs, HCQ@ROL\u2010LNPs had good targeting ability, showing enhanced therapeutic effect and low toxicity to other organs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Construction of HCQ@ROL\u2010LNPs delivery system lays a theoretical and experimental foundation for the treatment of liver fibrosis and promotes the development of clinical therapeutic drugs for liver diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/liv.15915","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T06:19:25Z","timestamp":1712902765000},"page":"1937-1951","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Targeted inhibition of autophagy in hepatic stellate cells by hydroxychloroquine: An effective therapeutic approach for the treatment of liver fibrosis"],"prefix":"10.1111","volume":"44","author":[{"given":"Li\u2010Shuang","family":"Hou","sequence":"first","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Pei","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao\u2010Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie\u2010Hua","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy Fourth Military Medical University  Xi'an 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