{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:29:53Z","timestamp":1772990993256,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>\n                      Hepatitis C virus (HCV) vaccines are urgently needed to achieve WHO's goal for the elimination of viral hepatitis by 2030. The lack of suitable animal models for evaluating vaccine efficacy has greatly hindered the development of HCV vaccines. By using the rat model chronically infected with rodent hepacivirus from\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Rattus norvegicus<\/jats:italic>\n                      <\/jats:styled-content>\n                      (RHV\u2010rn1), a hepacivirus homologously close to HCV as a surrogate model of HCV infection, we assessed the protective effectiveness of the RHV\u2010rn1 vaccine Sad23L\u2010RHVns.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Sad23L\u2010RHVns vaccine was constructed with the nonstructural proteins (NS) 3\u20135B genes of RHV\u2010rn1. SD rats were immunised with Sad23L\u2010RHVns by prime or prime\u2010boost regimen via intramuscular injection, then challenged 4\u2009weeks post vaccination by RHV\u2010rn1. A part of the rats were rechallenged with a variant 15\u2009weeks post the first challenge of RHV\u2010rn1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The specific T\u2010cell responses to NS3\u20105B antigens were induced by prime immunisation, which were significantly enhanced by boost vaccination. The inoculated rats and controls were challenged by wild\u2010type RHV\u2010rn1, of all the primed and control rats having persistently high levels of viremia, whereas 7 of 9 (77.8%) boosted rats cleared RHV\u2010rn1 infection. Interestingly, the resolver acquired immune protection against re\u2010challenging with variant and showed significantly higher T\u2010cell responses than the nonresolver in 25\u2009weeks post rechallenge.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Sad23L\u2010RHVns with prime\u2010boost regimen protected 77.8% of rats against wild\u2010type RHV\u2010rn1 infection, and resolvers showed high levels and maintenance of T cell immunity against the variant. Our findings that maintenance of effective T cell immunity is required for RHV\u2010rn1 resolution may provide insight to develop the HCV vaccine in humans.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/liv.70045","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T08:29:53Z","timestamp":1742200193000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Protection of Novel Adenovirus Vectored Vaccine in Rats Against Wild\u2010Type Hepacivirus and Variant Infections"],"prefix":"10.1111","volume":"45","author":[{"given":"Shengxue","family":"Luo","sequence":"first","affiliation":[{"name":"Institute of Clinical Blood Transfusion Guangzhou Blood Center  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qitao","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine School of Laboratory Medicine and Biotechnology, Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaolan","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Shenzhen Third People's Hospital  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine School of Laboratory Medicine and Biotechnology, Southern Medical University  Guangzhou China"},{"name":"Guangzhou Bai Rui Kang (BRK) Biological Science and Technology Limited Company  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine School of Laboratory Medicine and Biotechnology, Southern Medical University  Guangzhou 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