{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T10:44:11Z","timestamp":1775558651176,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Hepatitis B virus (HBV) remains a major global health problem. HBV DNA can be integrated into the human chromosomes. The integration in young cirrhotic chronic hepatitis B children has not been explored. This study aims to investigate HBV DNA integration in early childhood cirrhosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Biopsy liver specimens from cirrhotic and matched non\u2010cirrhotic chronic hepatitis B children were collected. HBV DNA integration was detected through targeted HBV DNA fragment capture sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Twenty cirrhotic and 20 non\u2010cirrhotic children with chronic hepatitis B were included in the study. The cirrhotic group included 14 males and 6 females, and the non\u2010cirrhotic group included 13 males and 7 females. Compared to non\u2010cirrhotic children, cirrhotic children had lower serum HBsAg quantification (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.001). The median number of HBV integrants in the cirrhotic group was 59 and that in the non\u2010cirrhotic group was 98. No significant difference existed between the two groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.529). In the multivariate linear regression analysis, serum HBV DNA level was correlated with the number of HBV integrants (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001,\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u20090.322). Six differential intragenic high\u2010frequency viral integration sites in cirrhotic children were revealed, all of which have protein\u2010coding functions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Several frequently integrated genes were observed in early childhood cirrhosis. Detailed associations between genetic alterations induced by HBV integration and early childhood cirrhosis need further exploration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/liv.70080","type":"journal-article","created":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T15:00:19Z","timestamp":1743087619000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genomic Integration of Hepatitis B Virus Into Human Hepatocytes in Early Childhood Cirrhosis"],"prefix":"10.1111","volume":"45","author":[{"given":"Ying","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory 962nd Hospital of PLA Joint Logistic Support Force  Harbin Heilongjiang Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4404-2766","authenticated-orcid":false,"given":"Yi","family":"Dong","sequence":"additional","affiliation":[{"name":"The Fifth Medical Center (formerly Beijing 302 Hospital), Chinese PLA General Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shizhang","family":"Wei","sequence":"additional","affiliation":[{"name":"Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Gao","sequence":"additional","affiliation":[{"name":"The Fifth Medical Center (formerly Beijing 302 Hospital), Chinese PLA General Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weijie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Beijing Ditan Hospital, Capital Medical University  Beijing 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