{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T02:40:43Z","timestamp":1788489643780,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82472167"],"award-info":[{"award-number":["82472167"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Metabolic dysfunction\u2013associated steatohepatitis (MASH)\u2010related fibrosis plays an important role in MASH prognosis; however, the underlying mechanism remains unknown. Here, we explored the involvement of miR\u2010210\u20103p in MASH\u2010associated fibrosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We examined miR\u2010210\u20103p expression in patients with MASH, with or without fibrosis using microarray analysis. miR\u2010210\u20103p expression both in\u00a0vivo and in\u00a0vitro was validated using real\u2010time quantitative reverse transcription PCR (RT\u2010qPCR). Target genes and mechanisms were explored using western blotting, dual luciferase assay and immunofluorescence staining. Ferroptosis was assessed based on the levels of malondialdehyde (MDA), glutathione (GSH), iron and ferroptosis\u2010related protein.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      miR\u2010210\u20103p decreased significantly in mice fed methionine\u2010choline\u2010deficient (MCD)\u2010fed and in those fed a high\u2010fat diet (HFD)\u2009+\u2009CCL\n                      <jats:sub>4<\/jats:sub>\n                      diets. Palmitic acid (PA)\u2010stimulated LX2 and primary hepatic stellate cells showed miR\u2010210\u20103p downregulation, consistent with the microarray results. miR\u2010210\u20103p overexpression alleviated MASH\u2010related fibrosis by inducing ferroptosis in hepatic stellate cells. Iron\u2013sulfur cluster assembly enzyme (ISCU) was validated as the downstream target of miR\u2010210\u20103p and its overexpression reversed miR\u2010210\u20103p\u2010induced ferroptosis. Overall, the ISCU\u2010IRP1\u2010CD71 axis is vital to miR\u2010210\u20103p\u2010induced ferroptosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>miR\u2010210\u20103p expression is decreased in MASH\u2010related fibrosis and is involved in ferroptosis by targeting ISCU.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/liv.70282","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T14:51:53Z","timestamp":1755528713000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["miR\u2010210\u20103p Ameliorates Metabolic Dysfunction\u2013Associated Steatohepatitis\u2010Related Fibrosis by Targeting ISCU and Induces Ferroptosis"],"prefix":"10.1111","volume":"45","author":[{"given":"Zixing","family":"Dai","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhua","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6294-9334","authenticated-orcid":false,"given":"Qingling","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuwen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengfan","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases The First Affiliated Hospital of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xizhi","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases The First Affiliated Hospital of Nanjing Medical University  Nanjing 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