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Here, it is demonstrated that integrin \u03b21 (ITG\u03b21, CD29) expression is upregulated by Tregs, enhancing cell adhesion and driving the malignant transformation of MASLD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A MASLD mouse model was established via high\u2010fat diet (HFD) and 2\u2010acetylamino fluorene (2\u2010AAF). Single\u2010cell RNA sequencing (scRNA\u2010seq) and flow cytometry were employed to quantitatively analyse the distribution of T lymphocytes and their subsets in the livers of MASLD mice, with particular focus on the Treg subset. Subsequently, a Treg subset with high CD29 expression was identified through unsupervised clustering analysis. Further validation using single\u2010cell sequencing data and in\u00a0vitro functional assays demonstrated that Tregs promote cell adhesion by upregulating CD29, thereby driving the progression of MASH to HCC. Finally, the effect of CD29 knockdown on the Tregs\u2010induced malignant transformation of MASLD was studied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      MASLD mice showed reduced hepatic T lymphocytes, imbalanced CD8\n                      <jats:sup>+<\/jats:sup>\n                      \/CD4\n                      <jats:sup>+<\/jats:sup>\n                      T ratios, and increased Tregs despite lower CD4\n                      <jats:sup>+<\/jats:sup>\n                      T proportions. Two Treg subsets were identified, one with high CD29 that was overexpressed in HCC; Tregs promoted cell adhesion via CD29 to drive MASH\u2010HCC transition, and CD29 knockdown attenuated this effect.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Tregs promote cell adhesion through CD29 upregulation, inducing the malignant transformation of MASLD. Targeting CD29 may offer a potential strategy for preventing HCC in patients with MASH.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/liv.70421","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:25:01Z","timestamp":1762507501000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Tregs with High\n                    <scp>CD29<\/scp>\n                    Expression Promote Cell Adhesion and Contribute to the Malignant Transformation of\n                    <scp>MASLD<\/scp>"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2792-2818","authenticated-orcid":false,"given":"Yuming","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Immunology Medical School of Nantong University  Nantong China"},{"name":"Department of Pathology The Second Affiliated Hospital of Soochow University  Suzhou 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