{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T05:46:47Z","timestamp":1782452807430,"version":"3.54.5"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Chronic hepatitis D is the most severe form of viral hepatitis. Despite recommendations for systematic screening of HBsAg\u2010positive individuals, hepatitis D infection remains underdiagnosed. Standard HDV virological markers are classically assessed in serum or plasma specimens obtained through venous whole blood sampling. This approach is costly and challenging in resource\u2010limited settings. Dried blood spot (DBS) sampling offers a practical alternative for large\u2010scale screening, diagnosis, and monitoring of viral hepatitis. The goal of the study is to evaluate the performance of commercially available HDV diagnostic assays using DBS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Plasma and DBS\u2010collected whole blood specimens from individuals chronically infected with HBV or coinfected with HBV and HDV were analysed for HDV antibody detection, HDV RNA quantification, HBsAg levels, and HDV genotyping.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      HDV antibodies were reliably detected in DBS after threshold adjustment, with the LIAISON XL Murex Anti\u2010HDV assay showing superior sensitivity (99.3%) compared to HDV Ab DIA.PRO (90.3%), while both assays exhibited excellent specificity. HDV RNA was quantifiable in 86.8% of DBS specimens from patients with active infection, although levels were about 1.5 log\n                      <jats:sub>10<\/jats:sub>\n                      lower than plasma, showing a strong correlation (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.786;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). HBsAg was detectable and quantifiable in all DBS samples, correlating closely with plasma values (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.98). DBS\u2010based HDV genotyping was successful in 84.8% of samples and concordant with plasma results.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>DBS collection of whole blood from DBS appears to be a promising and practical alternative to conventional venous blood sampling for HDV screening, diagnosis, and monitoring.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/liv.70759","type":"journal-article","created":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T04:09:48Z","timestamp":1781928588000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dried Blood Spot: A Tool to Ensure Broad Access to Hepatitis D Screening, Diagnosis, and Treatment Monitoring"],"prefix":"10.1111","volume":"46","author":[{"given":"Rola","family":"Matar","sequence":"first","affiliation":[{"name":"Department of Virology French National Reference Center for Hepatitis B, C and D Viruses, H\u00f4pital Henri Mondor (AP\u2010HP), Universit\u00e9 Paris\u2010Est  Cr\u00e9teil France"},{"name":"\u201cTeam Viruses, Hepatology, Cancer\u201d Institut de Recherche Biom\u00e9dicale, INSERM U955  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Roudot\u2010Thoraval","sequence":"additional","affiliation":[{"name":"\u201cTeam Viruses, Hepatology, Cancer\u201d Institut de Recherche Biom\u00e9dicale, INSERM U955  Cr\u00e9teil France"},{"name":"Department of Hepatology H\u00f4pital Henri Mondor (AP\u2010HP)  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Ortonne","sequence":"additional","affiliation":[{"name":"Department of Virology French National Reference Center for Hepatitis B, C and D Viruses, H\u00f4pital Henri Mondor (AP\u2010HP), Universit\u00e9 Paris\u2010Est  Cr\u00e9teil France"},{"name":"\u201cTeam Viruses, Hepatology, Cancer\u201d Institut de Recherche Biom\u00e9dicale, INSERM U955  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"Garrigou","sequence":"additional","affiliation":[{"name":"Department of Virology French National Reference Center for Hepatitis B, C and D Viruses, H\u00f4pital Henri Mondor (AP\u2010HP), Universit\u00e9 Paris\u2010Est  Cr\u00e9teil France"},{"name":"\u201cTeam Viruses, Hepatology, Cancer\u201d Institut de Recherche Biom\u00e9dicale, INSERM U955  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Soulier","sequence":"additional","affiliation":[{"name":"Department of Virology French National Reference Center for Hepatitis B, C and D Viruses, H\u00f4pital Henri Mondor (AP\u2010HP), Universit\u00e9 Paris\u2010Est  Cr\u00e9teil France"},{"name":"\u201cTeam Viruses, Hepatology, Cancer\u201d Institut de Recherche Biom\u00e9dicale, INSERM U955  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Cappy","sequence":"additional","affiliation":[{"name":"Department of Virology French National Reference Center for Hepatitis B, C and D Viruses, H\u00f4pital Henri Mondor (AP\u2010HP), Universit\u00e9 Paris\u2010Est  Cr\u00e9teil France"},{"name":"\u201cTeam Viruses, Hepatology, Cancer\u201d Institut de Recherche Biom\u00e9dicale, INSERM U955  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7738-5373","authenticated-orcid":false,"given":"Daniel","family":"Candotti","sequence":"additional","affiliation":[{"name":"Department of Virology French National Reference Center for Hepatitis B, C and D Viruses, H\u00f4pital Henri Mondor (AP\u2010HP), Universit\u00e9 Paris\u2010Est  Cr\u00e9teil France"},{"name":"\u201cTeam Viruses, Hepatology, Cancer\u201d Institut de Recherche Biom\u00e9dicale, INSERM U955  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Ingiliz","sequence":"additional","affiliation":[{"name":"\u201cTeam Viruses, Hepatology, Cancer\u201d Institut de Recherche Biom\u00e9dicale, INSERM U955  Cr\u00e9teil France"},{"name":"Department of Hepatology H\u00f4pital Henri Mondor (AP\u2010HP)  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0745-7559","authenticated-orcid":false,"given":"Jean\u2010Michel","family":"Pawlotsky","sequence":"additional","affiliation":[{"name":"Department of Virology French National Reference Center for Hepatitis B, C and D Viruses, H\u00f4pital Henri Mondor (AP\u2010HP), Universit\u00e9 Paris\u2010Est  Cr\u00e9teil France"},{"name":"\u201cTeam Viruses, Hepatology, Cancer\u201d Institut de Recherche Biom\u00e9dicale, INSERM U955  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Leroy","sequence":"additional","affiliation":[{"name":"\u201cTeam Viruses, Hepatology, Cancer\u201d Institut de Recherche Biom\u00e9dicale, INSERM U955  Cr\u00e9teil France"},{"name":"Department of Hepatology H\u00f4pital Henri Mondor (AP\u2010HP)  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6913-9017","authenticated-orcid":false,"given":"St\u00e9phane","family":"Chevaliez","sequence":"additional","affiliation":[{"name":"Department of Virology French National Reference Center for Hepatitis B, C and D Viruses, H\u00f4pital Henri Mondor (AP\u2010HP), Universit\u00e9 Paris\u2010Est  Cr\u00e9teil France"},{"name":"\u201cTeam Viruses, Hepatology, Cancer\u201d Institut de Recherche Biom\u00e9dicale, INSERM U955  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/HEP.0000000000000642"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2020.06.038"},{"key":"e_1_2_11_4_1","volume-title":"Guidelines for the Prevention, Diagnosis, Care and Treatment for People With Chronic Hepatitis B Infection","author":"Organization WH","year":"2024"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2023.05.001"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2025.03.018"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-015-9675-4"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3138\/canlivj-2025-0012-e"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29800"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvh.13779"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.16031"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23407"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0b013e3181edaaf5"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-60703-1"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25485"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2018.10.012"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkae418"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-82-11-2709"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29574"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.14309\/ajg.0000000000000954"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/HEP.0000000000000584"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2013.02.014"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2022.113412"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2023.116108"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/hsr2.229"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv423"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgph.0003320"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28772"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.15795"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2021.104932"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2019.12.028"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-018-3931-3"}],"container-title":["Liver International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/liv.70759","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/liv.70759","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/liv.70759","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T05:00:08Z","timestamp":1782450008000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/liv.70759"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,19]]},"references-count":31,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/liv.70759"],"URL":"https:\/\/doi.org\/10.1111\/liv.70759","archive":["Portico"],"relation":{},"ISSN":["1478-3223","1478-3231"],"issn-type":[{"value":"1478-3223","type":"print"},{"value":"1478-3231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,19]]},"assertion":[{"value":"2026-02-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70759"}}