{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:52:49Z","timestamp":1791557569926,"version":"4.3.4"},"reference-count":16,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:00:00Z","timestamp":1788566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:00:00Z","timestamp":1788566400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The differentiation between benign and malignant biliary strictures remains a significant clinical challenge. Recent studies have suggested that next generation sequencing (NGS) can improve the diagnostic accuracy. However, evidence on its performance in routine clinical practice is limited. Therefore, validation of the diagnostic value of NGS using real\u2010world clinical data is warranted. We compared the performance of NGS analysis of cholangiocellular carcinoma (CCC)\u2010associated mutations in endoscopic biopsies with that of histopathology, CA19\u20109 and cross\u2010sectional imaging. NGS showed higher sensitivity for malignancy (65%) compared to histopathology (52%) at similar specificity (96% vs. 100%). The combination of NGS and histopathology further increased sensitivity for malignancy to 78% (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.03) at similar specificity (96%). Our data provide support for the use of NGS in the diagnostic workup of biliary strictures.\n                  <\/jats:p>","DOI":"10.1111\/liv.70865","type":"journal-article","created":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T08:40:56Z","timestamp":1788597656000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Next Generation Sequencing for the Differentiation of Benign and Malignant Biliary Strictures"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0939-3573","authenticated-orcid":false,"given":"Antonia","family":"Gillmeister","sequence":"first","affiliation":[{"name":"Department of Medicine I, University Hospital Carl Gustav Carus Dresden Technische Universit\u00e4t Dresden (TUD)  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Husman","sequence":"additional","affiliation":[{"name":"Department of Medicine I, University Hospital Carl Gustav Carus Dresden Technische Universit\u00e4t Dresden (TUD)  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renate","family":"Schmelz","sequence":"additional","affiliation":[{"name":"Department of Medicine I, University Hospital Carl Gustav Carus Dresden Technische Universit\u00e4t Dresden (TUD)  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Br\u00fcckner","sequence":"additional","affiliation":[{"name":"Department of Medicine I, University Hospital Carl Gustav Carus Dresden Technische Universit\u00e4t Dresden (TUD)  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2421-6127","authenticated-orcid":false,"given":"Jochen","family":"Hampe","sequence":"additional","affiliation":[{"name":"Department of Medicine I, University Hospital Carl Gustav Carus Dresden Technische Universit\u00e4t Dresden (TUD)  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Aust","sequence":"additional","affiliation":[{"name":"Institute for Pathology, University Hospital Carl Gustav Carus Dresden Technische Universit\u00e4t Dresden (TUD)  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Zeissig","sequence":"additional","affiliation":[{"name":"Department of Medicine I, University Hospital Carl Gustav Carus Dresden Technische Universit\u00e4t Dresden (TUD)  Dresden Germany"},{"name":"Department of Visceral Surgery and Medicine, Inselspital \u2013 Bern University Hospital University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2015.182"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfma.2025.05.036"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22441"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2018-317817"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2021-325178"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.esmoop.2021.100275"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.961939"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13010039"},{"key":"e_1_2_12_10_1","first-page":"549","article-title":"Bile Cell\u2010Free DNA as a Novel and Powerful Liquid Biopsy for Detecting Somatic Variants in 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