{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T10:07:09Z","timestamp":1747822029932},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,4,17]],"date-time":"2014-04-17T00:00:00Z","timestamp":1397692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Erzincan University Scientific Research and Project Units","award":["11.02.09"],"award-info":[{"award-number":["11.02.09"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["LUTS"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>The objective of the present study was to evaluate anti\u2010inflammatory effects of hydroxyfasudil in a protamine sulfate (<jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content>) induced cystitis rat model. Additionally, we investigated prevention of bladder overactivity (<jats:styled-content style=\"fixed-case\">BO<\/jats:styled-content>), and tissue damage in these experiments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Animals were divided into four groups. In <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roups 1 and 2, chemical induced cystitis model was created by administrating intravesical <jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content> with <jats:styled-content style=\"fixed-case\">PE50<\/jats:styled-content> catheter by the transurethral route. In Group 1, Rho\u2010kinase inhibitor hydroxyfasudil was administered intaperitoneally, and in Group 2, subjects were administered a corresponding volume of saline in the same way. In <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup 3, vehicle was administered intravesically and hydroxyfasudil was administrated intraperitoneally. Group 4 was a control <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup, and the vehicle was administered intravesically and intraperitoneally. Micturition frequencies were recorded. Biochemical analyses were performed for oxidative stress, and pathological evaluations were investigated. In vitro contractions of bladder tissue strips were measured in tissue\u2010bath.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were significantly lower <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>ipid peroxidase levels and higher levels of <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>lutathione in <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup 1 than <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup 2 (<jats:italic>P<\/jats:italic>\u2009=\u20090.016, <jats:italic>P<\/jats:italic>\u2009=\u20090.001, respectively). There was generally more inflammation in <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup 2 than the other groups as determined by microscopy. There were significantly higher frequencies of micturition, lower volume, and mean voided maximum urine output after <jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content> administration in <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roups 1 and 2. In vitro contraction responses of bladder strips to potassium chloride and acetylcholine were statistically higher in <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup 2 than <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roups 1 and 3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Significant reduction of inflammation by affecting the anti\u2010oxidant defense systems was provided by hydroxyfasudil. Decreased <jats:italic>in vitro<\/jats:italic> responses to contractions of bladder smooth muscle strips were obtained. Hydroxyfasudil may be a potential new therapeutic option for inflammation and <jats:styled-content style=\"fixed-case\">BO<\/jats:styled-content>, in rat bladder.<\/jats:p><\/jats:sec>","DOI":"10.1111\/luts.12058","type":"journal-article","created":{"date-parts":[[2014,4,17]],"date-time":"2014-04-17T04:22:51Z","timestamp":1397708571000},"page":"108-114","source":"Crossref","is-referenced-by-count":6,"title":["Impact of <scp>R<\/scp>ho\u2010Kinase Inhibitor Hydroxyfasudil in Protamine Sulphate Induced Cystitis Rat Bladder"],"prefix":"10.1111","volume":"7","author":[{"given":"Yigit","family":"AKIN","sequence":"first","affiliation":[{"name":"Department of Urology Erzincan University School of Medicine  Erzincan Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aliseydi","family":"BOZKURT","sequence":"additional","affiliation":[{"name":"Department of Urology Erzincan University School of Medicine  Erzincan Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huseyin S.","family":"EROL","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Ataturk University School of Veterinary Medicine  Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mesut","family":"HALICI","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Ataturk University School of Veterinary Medicine  Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fikret","family":"CELEBI","sequence":"additional","affiliation":[{"name":"Department of Physiology Ataturk University School of Veterinary Medicine  Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kubra A. 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