{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T15:29:12Z","timestamp":1758814152383,"version":"3.37.3"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,9,18]],"date-time":"2014-09-18T00:00:00Z","timestamp":1410998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100004324","name":"Astellas Pharma Inc.","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100004324","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["LUTS"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To clarify the pathophysiological factor underlying neural crosstalk among pelvic organs, we investigated the possible role of nerve growth factor (<jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>) in the neural crosstalk between the testes and urinary bladder.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Nerve growth factor (10, 30, and 100\u2009\u00b5g\/mL) or saline was injected into the testes of male <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>istar rats. The change in bladder capacity via cystometry and duration of spontaneous scratching behavior induced by <jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content> in conscious rats was measured. The effects of pretreatment with capsaicin on <jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>\u2010induced changes in bladder capacity and behavior were examined. Further, we evaluated the effect of analgesics, indomethacin and morphine, and pretreatment with compound 48\/80 on <jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>\u2010induced scratching behavior to elucidate the mechanism of the behavior.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Injection of saline into the testes had no effect on bladder capacity or behavior. However, an injection of <jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content> (30 and 100\u2009\u00b5g\/mL) reduced bladder capacity, which was regarded as bladder overactivity, and evoked scratching behavior in a dose\u2010dependent manner. Pretreatment with capsaicin inhibited <jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content>\u2010induced bladder overactivity and scratching behavior. Neither indomethacin nor pretreatment with compound 48\/80 affected the scratching behavior, but morphine inhibited the behavior.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present study provides evidence of a possible new role of <jats:styled-content style=\"fixed-case\">NGF<\/jats:styled-content> in the testes regarding the activation of testicular primary afferent neurons mediated by capsaicin\u2010sensitive <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\u2010fibers, which evokes bladder overactivity via neural crosstalk between the testes and the urinary bladder as well as testicular pain.<\/jats:p><\/jats:sec>","DOI":"10.1111\/luts.12075","type":"journal-article","created":{"date-parts":[[2014,9,19]],"date-time":"2014-09-19T03:09:11Z","timestamp":1411096151000},"page":"62-67","source":"Crossref","is-referenced-by-count":4,"title":["Induction of Bladder Overactivity by Nerve Growth Factor in Testes in Rats: Possible Neural Crosstalk Between the Testes and Urinary Bladder"],"prefix":"10.1111","volume":"8","author":[{"given":"Katsuro","family":"YOSHIOKA","sequence":"first","affiliation":[{"name":"Pharmacology Research Labs, Astellas Pharma Inc.  Tsukuba\u2010shi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"TANAHASHI","sequence":"additional","affiliation":[{"name":"Pharmacology Research Labs, Astellas Pharma Inc.  Tsukuba\u2010shi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"TAKEDA","sequence":"additional","affiliation":[{"name":"Pharmacology Research Labs, Astellas Pharma Inc.  Tsukuba\u2010shi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriyuki","family":"MASUDA","sequence":"additional","affiliation":[{"name":"Pharmacology Research Labs, Astellas Pharma Inc.  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