{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:10:37Z","timestamp":1791846637360,"version":"4.3.4"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,3,28]],"date-time":"2016-03-28T00:00:00Z","timestamp":1459123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["LUTS"],"published-print":{"date-parts":[[2017,9]]},"abstract":"Objectives<\/jats:title>To investigate urothelial dysfunction, suburothelial inflammation, and muscarinic receptor expression in diabetic patients with overactive bladder (OAB<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>A total 19 patients with OAB<\/jats:styled-content> and diabetes mellitus (DM<\/jats:styled-content>), 14 OAB<\/jats:styled-content> patients without DM<\/jats:styled-content>, and 10 healthy control subjects were enrolled in the study. Immunofluorescence staining of E\u2010cadherin, zonula occludens\u20101 (ZO<\/jats:styled-content>\u20101), tryptase (mast cell activation), and apoptosis tests on the bladder urothelium and suburothelium were performed. Tissue muscarinic receptor M2<\/jats:styled-content>, M3<\/jats:styled-content>, and purinergic receptor P2X3<\/jats:styled-content> levels were measured by Western blotting. Video urodynamic studies were analyzed in relation to immunofluorescence and western blotting results.<\/jats:p><\/jats:sec>Results<\/jats:title>The mean age of the controls, OAB<\/jats:styled-content> patients without and with DM<\/jats:styled-content> were comparable (62.9 \u00b1 6.9, 58.4 \u00b1 21.3, and 68.9 \u00b1 11.3 years, respectively, P<\/jats:italic> = 0.25). E\u2010cadherin and mast cell expression in the bladder urothelium samples of the DM\u2010OAB<\/jats:styled-content> group and the OAB<\/jats:styled-content> group without DM<\/jats:styled-content> differed significantly from those of the control group (all P<\/jats:italic> < 0.05). However, immunofluorescence analyses including E\u2010cadherin, mast cells, apoptotic cell counts, and ZO<\/jats:styled-content>\u20101 were comparable between OAB<\/jats:styled-content> patients with and without DM<\/jats:styled-content>. In addition, M3<\/jats:styled-content> muscarinic protein expression in the bladders of OAB<\/jats:styled-content> patients with and without DM<\/jats:styled-content> was significantly higher than that in the control subjects. The video urodynamic parameters did not correlate with the immunofluorescence data, M2<\/jats:styled-content> and M3<\/jats:styled-content> muscarinic receptor data, or P2X3<\/jats:styled-content> protein expression.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Urothelial dysfunction and chronic suburothelial inflammation may contribute to the pathogeneses of OAB<\/jats:styled-content>. However, DM<\/jats:styled-content> does not aggravate the severity of urothelial inflammation in OAB<\/jats:styled-content> patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/luts.12126","type":"journal-article","created":{"date-parts":[[2016,3,28]],"date-time":"2016-03-28T13:26:47Z","timestamp":1459171607000},"page":"151-156","source":"Crossref","is-referenced-by-count":25,"title":["Urothelial Dysfunction and Chronic Inflammation in Diabetic Patients with Overactive Bladder"],"prefix":"10.1111","volume":"9","author":[{"given":"Chung\u2010Cheng","family":"WANG","sequence":"first","affiliation":[{"name":"Department of Urology En Chu Kong Hospital, College of Medicine, National Taiwan University Taipei Taiwan"},{"name":"Department of Biomedical Engineering Chung Yuan Christian University Chung\u2010Li Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hann\u2010Chorng","family":"KUO","sequence":"additional","affiliation":[{"name":"Department of Urology Buddhist Tzu Chi General Hospital, Tzu Chi University Hualien Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,3,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc07-0262"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11934-014-0440-3"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000133482.20685.d1"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(99)00871-X"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000056164"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00129.2012"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2012.02462.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.22494"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc08-9026"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.10052"},{"key":"e_1_2_7_12_1","first-page":"155","article-title":"Techniques of image analysis for quantitative immunohistochemistry","volume":"49","author":"Kaczmarek E","year":"2004","journal-title":"Rocz Akad Med Bialymst"},{"key":"e_1_2_7_13_1","first-page":"865463","article-title":"Evidence for bladder urothelial pathophysiology in functional bladder disorders","author":"Keay SK","year":"2014","journal-title":"Biomed Res Int"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00341.2003"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20869"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-013-1175-3"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2012.03095.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086463"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2007.05.114"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2012.12.021"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijcp.12196"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000136255.83054.0c"}],"container-title":["LUTS: Lower Urinary Tract Symptoms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fluts.12126","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fluts.12126","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/luts.12126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T12:22:50Z","timestamp":1696249370000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/luts.12126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,28]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/luts.12126"],"URL":"https:\/\/doi.org\/10.1111\/luts.12126","archive":["Portico"],"relation":{},"ISSN":["1757-5664","1757-5672"],"issn-type":[{"value":"1757-5664","type":"print"},{"value":"1757-5672","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,28]]}}}