{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T07:45:30Z","timestamp":1696319130570},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T00:00:00Z","timestamp":1462924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["LUTS"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>We investigated the role of bradykinin in urethral function by examining contractile responses in urethral smooth muscle strips isolated from humans and the intraurethral pressure in rats and dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The contractile responses of human urethral tissue for bradykinin (0.01\u201310 \u00b5mol\/L) were examined, and changes in intraurethral pressure induced by bradykinin (0.003\u201310 \u00b5g\/kg) in anesthetized rats or dogs were measured. In addition, the effects of pretreatment with the bradykinin <jats:styled-content style=\"fixed-case\">B2<\/jats:styled-content> receptor antagonist <jats:styled-content style=\"fixed-case\">FK3657<\/jats:styled-content> were also examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In smooth muscle strips obtained from human urethra, bradykinin induced contraction, which was inhibited by <jats:styled-content style=\"fixed-case\">FK3657<\/jats:styled-content> in a concentration\u2010dependent manner. In anesthetized rats and dogs, intravenously administered bradykinin dose\u2010dependently increased intraurethral pressure. <jats:styled-content style=\"fixed-case\">FK3657<\/jats:styled-content> shifted the intraurethral pressure dose\u2010response curve for bradykinin to the right in rats. The bradykinin\u2010induced elevation of intraurethral pressure was also dose\u2010dependently inhibited by <jats:styled-content style=\"fixed-case\">FK3657<\/jats:styled-content> in dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present study provides evidence that bradykinin elicits urethral smooth muscle contraction via the bradykinin <jats:styled-content style=\"fixed-case\">B2<\/jats:styled-content> receptor, suggesting the potential utility of this receptor as a novel target for the treatment of voiding dysfunction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/luts.12131","type":"journal-article","created":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T15:09:20Z","timestamp":1462979360000},"page":"187-191","source":"Crossref","is-referenced-by-count":0,"title":["Physiological Roles of Bradykinin and Involvement of Bradykinin <scp>B2<\/scp> Receptor in Urethral Function in Humans and Animals"],"prefix":"10.1111","volume":"9","author":[{"given":"Kenichiro","family":"FUJIMOTO","sequence":"first","affiliation":[{"name":"Drug Discovery Research Astellas Pharma Inc.  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