{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T01:20:28Z","timestamp":1770686428938,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T00:00:00Z","timestamp":1560729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["LUTS"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study investigated the effects of tadalafil on the urethra and detrusor in the initial phase of diabetes in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010six female Sprague\u2010Dawley rats were assigned to a non\u2010diabetes (ND), diabetes (D), or tadalafil\u2010treated diabetes (DT) group (n = 12 per group), with the DT group receiving oral tadalafil (2\u2009mg\/kg\/d) for 7 days before the experiments. Seven weeks after diabetes induction (by a single intraperitoneal injection of streptozotocin), urethral and intravesical pressure were simultaneously recorded in vivo, whereas responses of detrusor strips to potassium chloride (30\u2009mM), electrical field stimulation (EFS) and carbachol were measured in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The intravesical pressure at which the urethra started to relax was significantly lower in the DT than D group (mean [\u00b1 s.d.] 18.9\u2009\u00b1\u20092.9 vs 29.1\u2009\u00b1\u20096.6 cm H<jats:sub>2<\/jats:sub>O; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05). In addition, the reduction in urethral pressure was significantly larger in the DT than D group (\u221210.9\u2009\u00b1\u20094.0 vs \u22124.0\u2009\u00b1\u20092.9 cm H<jats:sub>2<\/jats:sub>O; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05). Detrusor stimulation revealed that the mean contractile responses to EFS and carbachol were significantly lower in the ND and DT groups than in the D group (120.7\u2009\u00b1\u200926.5% and 130.8\u2009\u00b1\u200915.8% vs 200.1\u2009\u00b1\u200947.9% of the 30\u2009mM KCl\u2010induced contraction, respectively, in response to 50\u2009Hz EFS [<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05]; 211.1\u2009\u00b1\u200935.4% and 208.4\u2009\u00b1\u200925.3% vs 425.7\u2009\u00b1\u2009125.0% of the 30\u2009mM KCl\u2010induced contraction, respectively, in response to 10<jats:sup>\u22123<\/jats:sup> M carbachol [<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05]).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Tadalafil restored urethral relaxation function and detrusor responses to EFS and carbachol during the initial phase of diabetes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/luts.12272","type":"journal-article","created":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T17:55:22Z","timestamp":1560794122000},"page":"241-247","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Tadalafil, a phosphodiesterase type 5 inhibitor, restores urethra and detrusor function in the initial phase of diabetes in rats"],"prefix":"10.1111","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6056-3820","authenticated-orcid":false,"given":"Daisuke","family":"Gotoh","sequence":"first","affiliation":[{"name":"Department of Urology Nara Medical University  Kashihara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2870-0466","authenticated-orcid":false,"given":"Kazumasa","family":"Torimoto","sequence":"additional","affiliation":[{"name":"Department of Urology Nara Medical University  Kashihara 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