{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:21:34Z","timestamp":1792639294025,"version":"4.5.0"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T00:00:00Z","timestamp":1561680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["LUTS"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to determine whether aging\u2010related detrusor underactivity (DU) involves a decrease in 5\u2010hydroxytryptamine (5\u2010HT\u2010positive)\u2010expressing urethral cells and whether 5\u2010HT stimulation of urethral sensory fibers improves detrusor function. Cystometries were performed in young (6 months) and aged (18\u201324\u2009months) female Wistar rats. Aged rats with voiding contractions (VC) that were 2SD below the mean of those in young rats were considered to have DU. Bladder voiding efficiency (BVE) was calculated during saline or 5\u2010HT solution cystometries. Rats were perfusion\u2010fixed with a fixative solution (paraformaldehyde, PFA 4%) through the circulatory system and the urethra sectioned to count the number of 5\u2010HT\u2010immunoreactive (IR) cells. Isovolumetric cystometry was performed while irrigating the urethra with saline or 5\u03bcM\u2010HT solution. Two\u2010tailed unpaired <jats:italic>t<\/jats:italic> tests were used to determine the significance of differences. In aged DU rats, the mean (\u00b1SD) VC frequency was 0.24\u2009\u00b1\u20090.07 per minute, with an amplitude of 15\u2009\u00b1\u20093 cm H<jats:sub>2<\/jats:sub>O. The mean (\u00b1SD) number of 5\u2010HT\u2010IR cells in the urethra of aged DU and young rats was 90\u2009\u00b1\u200911 and 182\u2009\u00b1\u200925, respectively (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). 5\u2010HT improved the mean (\u00b1SD) BVE of aged DU rats from 49\u2009\u00b1\u20093% to 78\u2009\u00b1\u20092% (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). In isovolumetric cystometries, detrusor pressure during irrigation of the urethra with saline was 18\u2009\u00b1\u20091 cm H<jats:sub>2<\/jats:sub>O, compared with 39\u2009\u00b1\u20092 cm H<jats:sub>2<\/jats:sub>O during irrigation with 5\u2010HT solution (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). In rats, DU associated with aging is accompanied by a decrease in the number of 5\u2010HT\u2010positive cells. The results suggest that decreased 5\u2010HT availability decreases urethral sensory fiber excitation, leading to a decrease the number of effective VC.<\/jats:p>","DOI":"10.1111\/luts.12273","type":"journal-article","created":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T08:53:49Z","timestamp":1561712029000},"page":"248-254","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Underactive bladder in aging rats is associated with a reduced number of serotonin\u2010expressing cells in the urethra and is improved by serotonin application to the urethra"],"prefix":"10.1111","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3286-0262","authenticated-orcid":false,"given":"Ana","family":"Coelho","sequence":"first","affiliation":[{"name":"Department of Biomedicine \u2013 Unit of Experimental Biology Faculty of Medicine of University of Porto  Porto Portugal"},{"name":"I3S \u2013 Institute of Investigation and Innovation in Health University of Porto  Porto Portugal"},{"name":"IBMC \u2013 Institute of Cellular and Molecular Biology University of Porto  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7717-7790","authenticated-orcid":false,"given":"Ana","family":"Charrua","sequence":"additional","affiliation":[{"name":"Department of Biomedicine \u2013 Unit of Experimental Biology Faculty of Medicine of University of Porto  Porto Portugal"},{"name":"I3S \u2013 Institute of Investigation and Innovation in Health University of Porto  Porto Portugal"},{"name":"IBMC \u2013 Institute of Cellular and Molecular Biology University of Porto  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biomedicine \u2013 Unit of Experimental Biology Faculty of Medicine of University of Porto  Porto Portugal"},{"name":"I3S \u2013 Institute of Investigation and Innovation in Health University of Porto  Porto Portugal"},{"name":"IBMC \u2013 Institute of Cellular and Molecular Biology University of Porto  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Cavaleiro","sequence":"additional","affiliation":[{"name":"Department of Biomedicine \u2013 Unit of Experimental Biology Faculty of Medicine of University of Porto  Porto Portugal"},{"name":"I3S \u2013 Institute of Investigation and Innovation in Health University of Porto  Porto Portugal"},{"name":"IBMC \u2013 Institute of Cellular and Molecular Biology University of Porto  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9lia D.","family":"Cruz","sequence":"additional","affiliation":[{"name":"Department of Biomedicine \u2013 Unit of Experimental Biology Faculty of Medicine of University of Porto  Porto Portugal"},{"name":"I3S \u2013 Institute of Investigation and Innovation in Health University of Porto  Porto Portugal"},{"name":"IBMC \u2013 Institute of Cellular and Molecular Biology University of Porto  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Cruz","sequence":"additional","affiliation":[{"name":"I3S \u2013 Institute of Investigation and Innovation in Health University of Porto  Porto Portugal"},{"name":"IBMC \u2013 Institute of Cellular and Molecular Biology University of Porto  Porto Portugal"},{"name":"Department of Surgery Faculty of Medicine of University of Porto  Porto Portugal"},{"name":"Department of Urology Hospital Centre S. Jo\u00e3o  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2013.10.015"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20798"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)35867-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfma.2017.09.006"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/003655901750174872"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.23590"},{"key":"e_1_2_6_8_1","first-page":"335","article-title":"Urethral sphincters response to cavernosus muscles stimulation with identification of cavernoso\u2010urethral reflex","volume":"51","author":"Shafik A","year":"2005","journal-title":"Syst Biol Reprod Med."},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2008.07752.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-003-0340-5"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(00)70328-0"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP274191"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12919"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOU.0000000000000361"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-016-1504-x"},{"key":"e_1_2_6_16_1","first-page":"624","article-title":"The laboratory rat: relating tts age with human's","volume":"4","author":"Pallav S","year":"2013","journal-title":"Int J Prev Med"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOU.0000000000000381"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.4111\/icu.2017.58.4.247"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.5213\/inj.1630504.252"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00496-1"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.107.125435"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/35039519"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2016.05.030"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.08.010"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-20406-0"}],"container-title":["LUTS: Lower Urinary Tract Symptoms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fluts.12273","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/luts.12273","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/luts.12273","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/luts.12273","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T01:15:12Z","timestamp":1694308512000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/luts.12273"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,28]]},"references-count":24,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1111\/luts.12273"],"URL":"https:\/\/doi.org\/10.1111\/luts.12273","archive":["Portico"],"relation":{},"ISSN":["1757-5664","1757-5672"],"issn-type":[{"value":"1757-5664","type":"print"},{"value":"1757-5672","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,28]]},"assertion":[{"value":"2019-01-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}