{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:42:09Z","timestamp":1747186929989,"version":"3.40.5"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,8,20]],"date-time":"2019-08-20T00:00:00Z","timestamp":1566259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["LUTS"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To further explore the effects of a novel EP2 and EP3 dual agonist, ONO\u20108055, on detrusor contractility, we investigated the responses of bladder strips from sham and lumbar canal stenosis (LCS) rats to this agonist, its effects on lower urinary tract function in normal rats, and mRNA expression of <jats:italic>EP2<\/jats:italic> and <jats:italic>EP3<\/jats:italic> receptors in the sham and LCS rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The responses of bladder strips from sham and LCS rats to ONO\u20108055 were measured. The effects of ONO\u20108055 on LUT function of normal rats were investigated with awake cystometry and intraurethral perfusion pressure (Pura) measurements. The relative mRNA of bladder and urethral tissue of the sham and LCS rats was quantified using specific probes for <jats:italic>EP1<\/jats:italic>, <jats:italic>EP2<\/jats:italic>, <jats:italic>EP3<\/jats:italic>, and <jats:italic>EP4<\/jats:italic> genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with the vehicle, the muscle tensions of both the sham and LCS rats were significantly increased after adding this agonist. On awake cystometry of normal rats, bladder capacity and Pura were decreased in the ONO\u20108055 groups, but a statistically significant difference in mean changes was demonstrated only between the vehicle group and the group receiving the highest dose. Compared with the sham rats, mRNA expressions of the four EP receptors in the lower urinary tract of the LCS rats did not show a statistically significant difference.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This agonist did not augment bladder contractility or urethral relaxation in normal rats.<\/jats:p><\/jats:sec>","DOI":"10.1111\/luts.12284","type":"journal-article","created":{"date-parts":[[2019,8,20]],"date-time":"2019-08-20T13:18:22Z","timestamp":1566307102000},"page":"99-106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Further characterization of a novel EP2 and EP3 receptor dual agonist, ONO\u20108055, on lower urinary tract function in normal and lumbar canal stenosis rats"],"prefix":"10.1111","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0169-4783","authenticated-orcid":false,"given":"Noritoshi","family":"Sekido","sequence":"first","affiliation":[{"name":"Department of Urology School of Medicine, Faculty of Medicine, Toho University Ohashi Medical Center  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Kida","sequence":"additional","affiliation":[{"name":"Discovery Research Laboratories II Ono Pharmaceutical Co. Ltd.  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeya","family":"Otsuki","sequence":"additional","affiliation":[{"name":"Discovery Research Laboratories II Ono Pharmaceutical Co. Ltd.  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroko","family":"Mashimo","sequence":"additional","affiliation":[{"name":"Discovery Research Laboratories II Ono Pharmaceutical Co. Ltd.  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidekazu","family":"Matsuya","sequence":"additional","affiliation":[{"name":"Discovery Research Laboratories II Ono Pharmaceutical Co. Ltd.  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Okada","sequence":"additional","affiliation":[{"name":"Discovery Research Laboratories II Ono Pharmaceutical Co. Ltd.  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,20]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2013.10.015"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-43087-4_6"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharep.2016.03.007"},{"key":"e_1_2_13_5_1","first-page":"1190","article-title":"A novel animal model of underactive bladder: analysis of lower urinary tract function in a rat lumbar canal stenosis model","volume":"31","author":"Sekido N","year":"2012","journal-title":"NeurourolUrodyn"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2014.07.036"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2016.02.064"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/luts.12166"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.07.087"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(88)90221-X"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2006.09.076"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2006.01274.x"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0102644"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP085418"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.90273.2008"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00054.2008"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00275.x"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.01.131"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/luts.12077"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00270.2017"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2012.11096.x"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.09260.x"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2006.11.070"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00326.2011"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2008.05.007"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00271.2013"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-012-1088-9"}],"container-title":["LUTS: Lower Urinary Tract Symptoms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/luts.12284","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/luts.12284","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/luts.12284","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T14:35:52Z","timestamp":1694097352000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/luts.12284"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,20]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1111\/luts.12284"],"URL":"https:\/\/doi.org\/10.1111\/luts.12284","archive":["Portico"],"relation":{},"ISSN":["1757-5664","1757-5672"],"issn-type":[{"type":"print","value":"1757-5664"},{"type":"electronic","value":"1757-5672"}],"subject":[],"published":{"date-parts":[[2019,8,20]]},"assertion":[{"value":"2019-05-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}