{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:02:05Z","timestamp":1787770925884,"version":"build-2784847793"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T00:00:00Z","timestamp":1716336000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP17K12903"],"award-info":[{"award-number":["JP17K12903"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21K14820"],"award-info":[{"award-number":["JP21K14820"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["LUTS"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study evaluates the impact of equol, a metabolite of soy isoflavone, on bladder dysfunction in rats with bladder outlet obstruction (BOO). In addition, we investigate its potential as a neuroprotective agent for the obstructed bladder and discuss its applicability in managing overactive bladder (OAB).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eighteen male Sprague\u2013Dawley rats were divided into three groups (six rats per group) during the rearing period. The Sham and C\u2010BOO groups received an equol\u2010free diet, while the E\u2010BOO group received equol supplementation (0.25\u2009g\/kg). At 8\u2009weeks old, rats underwent BOO surgery, followed by continuous cystometry after 4\u2009weeks of rearing. The urinary oxidative stress markers (8\u2010hydroxy\u20102\u2032\u2010deoxyguanosine and malondialdehyde) were measured, and the bladder histology was analyzed using hematoxylin\u2013eosin, Masson's trichrome, and immunohistochemical staining (neurofilament heavy chain for myelinated nerves, peripherin for unmyelinated nerves, and malondialdehyde).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Equol reduced BOO\u2010induced smooth muscle layer fibrosis, significantly prolonged the micturition interval (C\u2010BOO: 193\u2009s, E\u2010BOO: 438\u2009s) and increased the micturition volume (C\u2010BOO: 0.54\u2009mL, E\u2010BOO: 1.02\u2009mL) compared to the C\u2010BOO group. Equol inhibited the increase in urinary and bladder tissue malondialdehyde levels. While the C\u2010BOO group exhibited reduced peripherin alone positive nerve fibers within the smooth muscle layer, equol effectively attenuated this decline.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Equol reduces lipid peroxidation and smooth muscle layer fibrosis in the bladder and exhibited neuroprotective effects on bladder nerves (peripheral nerves) and prevented the development of bladder dysfunction associated with BOO in rats. Consumption of equol is promising for the prevention of OAB associated with BOO.<\/jats:p><\/jats:sec>","DOI":"10.1111\/luts.12518","type":"journal-article","created":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T02:26:43Z","timestamp":1716431203000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Protective effect of equol intake on bladder dysfunction in a rat model of bladder outlet obstruction"],"prefix":"10.1111","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5075-5485","authenticated-orcid":false,"given":"Nozomu","family":"Miyazaki","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Medicine Fukushima Medical University  Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryota","family":"Katsura","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine Fukushima Medical University  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