{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T21:20:54Z","timestamp":1784928054586,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["LUTS"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The present study investigates the therapeutic potential of <jats:styled-content style=\"fixed-case\"><jats:italic>Cucurbita Pepo<\/jats:italic><\/jats:styled-content> L. Seeds' Oil (CPSO) extracted by petroleum ether on testosterone\u2010induced hyperplasia (BPH) in a castrated Wistar rat model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Animals underwent two stages of treatment (28\u2009days each): the testosterone treatment stage, to promote BPH, and the therapeutic stage, which consisted of an oral administration of CPSO. Forty\u2010eight Wistar rats were equally divided into six groups: the negative control; groups 2\u20136 underwent a bilateral orchidectomy (induced via the scrotal route), then were redistributed into the castrated\u2010control group and the castrated and testosterone\u2010treated groups BPH induction (10\u2009mg\/kg). Twenty\u2010eight days after BPH induction, the BPH rats were orally treated with Finasteride (FINA group: 5\u2009mg\/kg\/d) and CPSO (CPSO\u20101 group: 40\u2009mg\/kg\/d; CPSO\u20102 group: 80\u2009mg\/kg\/d) for 28 additional days. Plasma biochemical parameters, circulating testosterone (T) and prostate\u2010specific antigen (PSA), prostate oxidative markers, and inflammation were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CPSO administration exhibited a decrease in prostate weight, normalized the plasma total proteins, lipids, calcium and glucose, testosterone, and PSA levels, and attenuated the disturbances of liver and kidney functions in BPH rats. At the prostate level, CPSO showed a notably decreased prostate oxidation and inflammation. The histological study showed that CPSO restored prostatic lesions caused by BPH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>According to the present results, we suggest that CPSO could have a protective potential related to its proven anti\u2010oxidant, anti\u2010hyperplastic, and anti\u2010inflammatory properties on preventing testosterone\u2010induced BPH in castrated rats and might be used as an herbal remedy or nutraceutical agent in BPH management.<\/jats:p><\/jats:sec>","DOI":"10.1111\/luts.70024","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T08:15:17Z","timestamp":1754381717000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigating the Antihyperplastic, Anti\u2010Inflammatory and Antioxidant Properties of <scp><i>Cucurbita Pepo<\/i><\/scp> L. Seed Oil on Testosterone\u2010 Induced Benign Prostatic Hyperplasia (<scp>BPH<\/scp>) in Castrated Rat Model"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3094-1092","authenticated-orcid":false,"given":"Hajji","family":"Noura","sequence":"first","affiliation":[{"name":"Laboratory of Mycology, Pathology &amp; Biomarkers (LR16ES05), Department of Biology Faculty of Sciences of Tunis, University of Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8704-6006","authenticated-orcid":false,"given":"Kh\u00e9miri","family":"Ikram","sequence":"additional","affiliation":[{"name":"Laboratory of Mycology, Pathology &amp; Biomarkers (LR16ES05), Department of Biology Faculty of Sciences of Tunis, University of Tunis El Manar  Tunis 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