{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:24:16Z","timestamp":1791141856836,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,2,20]],"date-time":"2014-02-20T00:00:00Z","timestamp":1392854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":558,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Bartonella quintana<\/jats:italic> is transmitted by the infected faeces of body lice. Recently, this bacterium was detected in cat fleas (<jats:italic>Ctenocephalides felis<\/jats:italic>) and in two humans with chronic adenopathy whose only risk factor was contact with cat fleas. In this study, a total of 960 <jats:italic>C.\u00a0felis<\/jats:italic> were divided into 12 groups (2 control groups and 10 infected groups) each containing 80 fleas. The fleas were fed <jats:italic>B.\u00a0quintana<\/jats:italic>\u2010inoculated human blood at different dilutions (\u22483.6\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup> \u2212 8.4\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup> bacteria) for 4\u00a0days via an artificial membrane. Subsequently, all flea groups were fed uninfected blood until day 13 postinfection (dpi). On day 3 pi, <jats:italic>B.\u00a0quintana<\/jats:italic> was detected with two specific genes by quantitative PCR in 60\u2013100% of randomly chosen fleas per dilution: 52% (26\/50) in the infected fleas in Trial 1 and 90% (45\/50) of the fleas in Trial 2. <jats:italic>B.\u00a0quintana<\/jats:italic> was also identified by molecular and culture assays in flea faeces. The average number of <jats:italic>B.\u00a0quintana<\/jats:italic> as determined by q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> decreased until the 11th dpi and was absent in both trials at the 13th dpi. Bacteria were localized only in the flea gastrointestinal gut by specific immunohistochemistry. Our results indicate that cat fleas can acquire <jats:italic>B.\u00a0quintana<\/jats:italic> by feeding and release viable organisms into their faeces. Therefore, fleas may play a role as vectors of trench fever or other clinical manifestations that are caused by <jats:italic>B.\u00a0quintana<\/jats:italic>. However, the biological role of <jats:italic>C.\u00a0felis<\/jats:italic> in the transmission of <jats:italic>B.\u00a0quintana<\/jats:italic> under natural conditions is yet to be defined.<\/jats:p>","DOI":"10.1111\/mec.12663","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:48:00Z","timestamp":1389203280000},"page":"1204-1212","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Acquisition and excretion of <i><scp>B<\/scp>artonella quintana<\/i> by the cat flea, <i><scp>C<\/scp>tenocephalides felis felis<\/i>"],"prefix":"10.1111","volume":"23","author":[{"given":"Tahar","family":"Kernif","sequence":"first","affiliation":[{"name":"Aix Marseille Universit\u00e9 URMITE UM63 CNRS 7278 IRD 198 Inserm 1095 Marseille 13005 France"},{"name":"Institut Pasteur d'Alg\u00e9rie Algiers 16015 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