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Although of diverse origin, the persistence of\n                    <jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>\n                    s in aquatic environments is highly influenced by anthropic activities, allowing potential control actions in well\u2010studied environments. However, knowledge of abundance and space\u2013time distribution of\n                    <jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>\n                    s in ecosystems is still scarce. Using quantitative real\u2010time PCR, we investigated the presence and the abundance of twelve\n                    <jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>\n                    s (against tetracyclines, \u03b2\u2010lactams, aminoglycosides, quinolones and sulphonamides) at different sampling sites, depths and seasons, in Lake Maggiore, a large subalpine lake, and in the area of its watershed. We then evaluated the correlation between each\n                    <jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>\n                    and a number of ecological parameters in the water column in the deepest part of the lake. Our results suggest the constitutive presence of at least four\n                    <jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>\n                    s within the bacterial community with a high proportion of bacteria potentially resistant to tetracyclines and sulphonamides. The presence of these\n                    <jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>\n                    s was independent of the total bacterial density and temperature. The dynamics of\n                    <jats:italic>tet<\/jats:italic>\n                    (A) and sul\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\n                    <\/jats:italic>\n                    genes were, however, positively correlated with dissolved oxygen and negatively to chlorophyll a, suggesting that the resistant microbes inhabit specific niches. These observations indicate that the lake is a reservoir of antibiotic resistances, highlighting the need of a deeper understanding of the sources of\n                    <jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>\n                    s and the factors allowing their persistence in waters.\n                  <\/jats:p>","DOI":"10.1111\/mec.13293","type":"journal-article","created":{"date-parts":[[2015,6,28]],"date-time":"2015-06-28T04:43:00Z","timestamp":1435466580000},"page":"3888-3900","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":116,"title":["Constitutive presence of antibiotic resistance genes within the bacterial community of a large subalpine lake"],"prefix":"10.1111","volume":"24","author":[{"given":"Andrea","family":"Di Cesare","sequence":"first","affiliation":[{"name":"Microbial Ecology Group National Research Council \u2013 Institute of Ecosystem Study (CNR\u2010ISE) Largo Tonolli 50 28922 Verbania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ester M.","family":"Eckert","sequence":"additional","affiliation":[{"name":"Microbial Ecology Group National Research Council \u2013 Institute of Ecosystem Study (CNR\u2010ISE) Largo Tonolli 50 28922 Verbania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Teruggi","sequence":"additional","affiliation":[{"name":"Microbial Ecology Group National Research Council \u2013 Institute of Ecosystem Study (CNR\u2010ISE) Largo Tonolli 50 28922 Verbania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Fontaneto","sequence":"additional","affiliation":[{"name":"Microbial Ecology Group National Research Council \u2013 Institute of Ecosystem Study (CNR\u2010ISE) Largo Tonolli 50 28922 Verbania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Bertoni","sequence":"additional","affiliation":[{"name":"Microbial Ecology Group National Research Council \u2013 Institute of Ecosystem Study (CNR\u2010ISE) Largo Tonolli 50 28922 Verbania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiana","family":"Callieri","sequence":"additional","affiliation":[{"name":"Microbial Ecology Group National Research Council \u2013 Institute of Ecosystem Study (CNR\u2010ISE) Largo Tonolli 50 28922 Verbania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluca","family":"Corno","sequence":"additional","affiliation":[{"name":"Microbial Ecology Group National Research Council \u2013 Institute of Ecosystem Study (CNR\u2010ISE) Largo Tonolli 50 28922 Verbania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/es405348h"},{"key":"e_1_2_7_3_1","first-page":"209","volume-title":"OSMED","author":"AIFA","year":"2012"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4081\/jlimnol.2010.3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku079"},{"key":"e_1_2_7_6_1","unstructured":"ARCH\u2010Vet. 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