{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:09:28Z","timestamp":1761581368814},"reference-count":16,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2015,11,26]],"date-time":"2015-11-26T00:00:00Z","timestamp":1448496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"EC FP7 Marie Curie International Outgoing Fellowship","award":["302503"],"award-info":[{"award-number":["302503"]}]}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>Tibayrenc and Ayala raised several interesting objections to an opinion piece we recently published in <jats:italic>Molecular Ecology<\/jats:italic> (Ramirez &amp; Llewellyn 2014). Our piece examined the value of an alternative perspective to their theory of predominant clonal evolution (<jats:styled-content style=\"fixed-case\">PCE<\/jats:styled-content>) on the prevalence and importance of genetic exchange in parasitic protozoa. In particular, our aim was to establish whether population genetic signatures of clonality in parasites were representative of true biological\/evolutionary processes or artefacts of inadequate tools and inappropriate or inadequate sampling. We address Tibayrenc and Ayala's criticisms and make a detailed response. In doing so, we deny the consensus that Tibayrenc and Ayala claim around their views and dismiss much of the language which Tibayrenc and Ayala have introduced to this debate as either arbitrary or inaccurate. We strongly reject accusations that we misunderstood and misquoted the work of others. We do not think the <jats:styled-content style=\"fixed-case\">PCE<\/jats:styled-content> provides a useful framework for understanding existing parasite population structures. Furthermore, on the eve of the population genomic era, we strongly urge Tibayrenc and Ayala to wait for the forthcoming wealth of high\u2010resolution data before considering whether it is appropriate to refine or re\u2010iterate their <jats:styled-content style=\"fixed-case\">PCE<\/jats:styled-content> hypothesis.<\/jats:p>","DOI":"10.1111\/mec.13442","type":"journal-article","created":{"date-parts":[[2015,11,26]],"date-time":"2015-11-26T09:52:11Z","timestamp":1448531531000},"page":"5782-5784","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Response to Tibayrenc and Ayala: reproductive clonality in protozoan pathogens \u2013 truth or artefact?"],"prefix":"10.1111","volume":"24","author":[{"given":"J. D.","family":"Ram\u00edrez","sequence":"first","affiliation":[{"name":"Grupo de Investigaciones Microbiol\u00f3gicas \u2013 UR (GIMUR) Facultad de Ciencias Naturales y Matem\u00e1ticas Universidad del Rosario Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. S.","family":"Llewellyn","sequence":"additional","affiliation":[{"name":"Institute of Biodiversity Animal Health and Comparative Medicine University of Glasgow Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,11,26]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Molecular Markers, Natural History and Evolution","author":"Avise J","year":"2004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019751108"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001507"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811080106"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.10.4384"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-139"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000915"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12872"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2156-14-96"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004092"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2015.05.006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1212452109"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2013.03.007"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1502900112"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2015.05.006"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.13443"}],"container-title":["Molecular Ecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmec.13442","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mec.13442","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T09:12:59Z","timestamp":1696497179000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mec.13442"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,26]]},"references-count":16,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1111\/mec.13442"],"URL":"https:\/\/doi.org\/10.1111\/mec.13442","archive":["Portico"],"relation":{},"ISSN":["0962-1083","1365-294X"],"issn-type":[{"value":"0962-1083","type":"print"},{"value":"1365-294X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,26]]}}}