{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:24:14Z","timestamp":1791318254846,"version":"4.3.1"},"reference-count":60,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,5,18]],"date-time":"2016-05-18T00:00:00Z","timestamp":1463529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:p><jats:italic>Symbiodinium<\/jats:italic> are a diverse group of unicellular dinoflagellates that are important nutritional symbionts of reef\u2010building corals. <jats:italic>Symbiodinium<\/jats:italic> putative species (\u2018types\u2019) are commonly identified with genetic markers, mostly nuclear and chloroplast encoded ribosomal <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> regions. Population genetic analyses using microsatellite loci have provided insights into <jats:italic>Symbiodinium<\/jats:italic> biogeography, connectivity and phenotypic plasticity, but are complicated by: (i) a lack of consensus criteria used to delineate inter\u2010 vs. intragenomic variation within species; and (ii) the high density of <jats:italic>Symbiodinium<\/jats:italic> in host tissues, which results in single samples comprising thousands of individuals. To address this problem, Wham &amp; LaJeunesse (2016) present a method for identifying cryptic <jats:italic>Symbiodinium<\/jats:italic> species from microsatellite data based on correlations between allele size distributions and nongeographic genetic structure. Multilocus genotypes that potentially do not recombine in sympatry are interpreted as secondary \u2018species\u2019 to be discarded from downstream population genetic analyses. However, <jats:italic>Symbiodinium<\/jats:italic> species delineations should ideally incorporate multiple physiological, ecological and molecular criteria. This is because recombination tests may be a poor indicator of species boundaries in <jats:italic>Symbiodinium<\/jats:italic> due to their predominantly asexual mode of reproduction. Furthermore, discontinuous microsatellite allele sizes in sympatry may be explained by secondary contact between previously isolated populations and by mutations that occur in a nonstepwise manner. Limitations of using microsatellites alone to delineate species are highlighted in earlier studies that demonstrate occasional bimodal distributions of\u00a0allele sizes within <jats:italic>Symbiodinium<\/jats:italic> species and considerable allele size sharing among <jats:italic>Symbiodinium<\/jats:italic> species. We outline these issues and discuss the validity of reinterpretations of our previously published microsatellite data from <jats:italic>Symbiodinium<\/jats:italic> populations on the Great Barrier Reef (Howells <jats:italic>et\u00a0al<\/jats:italic>. 2013).<\/jats:p>","DOI":"10.1111\/mec.13631","type":"journal-article","created":{"date-parts":[[2016,5,18]],"date-time":"2016-05-18T14:29:00Z","timestamp":1463581740000},"page":"2719-2723","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Microsatellite allele sizes alone are insufficient to delineate species boundaries in <i>Symbiodinium<\/i>"],"prefix":"10.1111","volume":"25","author":[{"given":"E. J.","family":"Howells","sequence":"first","affiliation":[{"name":"Center for Genomics and Systems Biology New York University Abu Dhabi Saadiyat Island Abu Dhabi United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. L.","family":"Willis","sequence":"additional","affiliation":[{"name":"ARC Centre of Excellence for Coral Reef Studies and College of Marine and Environmental Sciences James Cook University Townsville Qld 4811 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. K.","family":"Bay","sequence":"additional","affiliation":[{"name":"Australian Institute of Marine Science PMB 3 MC Townsville Qld 4810 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J. 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