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We performed a metaproteomic study on the Abrolhos coral <jats:italic>Mussismilia braziliensis<\/jats:italic> to define the types of proteins expressed in healthy corals compared to <jats:styled-content style=\"fixed-case\">WPS<\/jats:styled-content>\u2010 and <jats:styled-content style=\"fixed-case\">BBD<\/jats:styled-content>\u2010affected corals. A total of 6363 <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> spectra were identified as 361 different proteins. Healthy corals had a set of proteins that may be considered markers of holobiont homoeostasis, including tubulin, histone, Rab family, ribosomal, peridinin\u2013chlorophyll a\u2010binding protein, F0F1\u2010type <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content> synthase, alpha\u2010<jats:styled-content style=\"fixed-case\">iG<\/jats:styled-content> protein, calmodulin and <jats:styled-content style=\"fixed-case\">ADP<\/jats:styled-content>\u2010ribosylation factor. <jats:italic>Cnidaria<\/jats:italic> proteins found in healthy <jats:italic>M.\u00a0braziliensis<\/jats:italic> were associated with <jats:italic>Cnidaria<\/jats:italic>\u2013<jats:italic>Symbiodinium<\/jats:italic> endosymbiosis and included chaperones (hsp70, hsp90 and calreticulin), structural and membrane modelling proteins (actin) and proteins with functions related to intracellular vesicular traffic (Rab7 and <jats:styled-content style=\"fixed-case\">ADP<\/jats:styled-content>\u2010ribosylation factor 1) and signal transduction (14\u20103\u20103 protein and calmodulin). <jats:styled-content style=\"fixed-case\">WPS<\/jats:styled-content> resulted in a clear shift in the predominance of proteins, from those related to aerobic nitrogen\u2010fixing bacteria (i.e. <jats:italic>Rhizobiales, Sphingomonadales<\/jats:italic> and <jats:italic>Actinomycetales<\/jats:italic>) in healthy corals to those produced by facultative\/anaerobic sulphate\u2010reducing bacteria (i.e. <jats:italic>Enterobacteriales<\/jats:italic>,<jats:italic> Alteromonadales<\/jats:italic>,<jats:italic> Clostridiales<\/jats:italic> and <jats:italic>Bacteroidetes<\/jats:italic>) in <jats:styled-content style=\"fixed-case\">WPS<\/jats:styled-content> corals. <jats:styled-content style=\"fixed-case\">BBD<\/jats:styled-content> corals developed a diverse community dominated by cyanobacteria and sulphur cycle bacteria. Hsp60, hsp90 and adenosylhomocysteinase proteins were produced mainly by cyanobacteria in <jats:styled-content style=\"fixed-case\">BBD<\/jats:styled-content> corals, which is consistent with elevated oxidative stress in hydrogen sulphide\u2010 and cyanotoxin\u2010rich environments. This study demonstrates the usefulness of metaproteomics for gaining better comprehension of coral metabolic status in health and disease, especially in reef systems such as the Abrolhos that are suffering from the increase in global and local threatening events.<\/jats:p>","DOI":"10.1111\/mec.13775","type":"journal-article","created":{"date-parts":[[2016,8,5]],"date-time":"2016-08-05T04:41:28Z","timestamp":1470372088000},"page":"4632-4644","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Metaproteomics reveals metabolic transitions between healthy and diseased stony coral <i>Mussismilia braziliensis<\/i>"],"prefix":"10.1111","volume":"25","author":[{"given":"Gizele D.","family":"Garcia","sequence":"first","affiliation":[{"name":"Instituto de Biologia Universidade Federal do Rio de Janeiro (UFRJ) Av. Carlos Chagas Fo. SN. 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