{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:01:25Z","timestamp":1790755285427,"version":"4.1.0"},"reference-count":90,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,3,23]],"date-time":"2018-03-23T00:00:00Z","timestamp":1521763200000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["We4641\/1\u20102"],"award-info":[{"award-number":["We4641\/1\u20102"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>On theoretical grounds, antagonistic co\u2010evolution between hosts and their parasites should be a widespread phenomenon but only received little empirical support so far. Consequently, the underlying molecular mechanisms and evolutionary steps remain elusive, especially in nonmodel systems. Here, we utilized the natural history of invasive parasites to document the molecular underpinnings of co\u2010evolutionary trajectories. We applied a dual\u2010species transcriptomics approach to experimental cross\u2010infections of blue mussel <jats:italic>Mytilus edulis<\/jats:italic> hosts and their invasive parasitic copepods <jats:italic>Mytilicola intestinalis<\/jats:italic> from two invasion fronts in the Wadden Sea. We identified differentially regulated genes from an experimental infection contrast for hosts (infected vs. control) and a sympatry contrast (sympatric vs. allopatric combinations) for both hosts and parasites. The damage incurred by <jats:italic>Mytilicola<\/jats:italic> infection and the following immune response of the host were mainly reflected in cell division processes, wound healing, apoptosis and the production of reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>). Furthermore, the functional coupling of host and parasite sympatry contrasts revealed the concerted regulation of chitin digestion by a Chitotriosidase 1 homolog in hosts with several cuticle proteins in the parasite. Together with the coupled regulation of <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> producers and antagonists, these genes represent candidates that mediate the different evolutionary trajectories within the parasite's invasion. The host\u2013parasite combination\u2010specific coupling of these effector mechanisms suggests that underlying recognition mechanisms create specificity and local adaptation. In this way, our study demonstrates the use of invasive species\u2019 natural history to elucidate molecular mechanisms of host\u2013parasite co\u2010evolution in the wild.<\/jats:p>","DOI":"10.1111\/mec.14541","type":"journal-article","created":{"date-parts":[[2018,2,17]],"date-time":"2018-02-17T14:16:51Z","timestamp":1518877011000},"page":"1505-1519","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Dual transcriptomics reveals co\u2010evolutionary mechanisms of intestinal parasite infections in blue mussels <i>Mytilus edulis<\/i>"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1022-8012","authenticated-orcid":false,"given":"Marieke E.","family":"Feis","sequence":"first","affiliation":[{"name":"Department Coastal Ecology Wadden Sea Station Sylt Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research List\/Sylt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1297-4086","authenticated-orcid":false,"given":"Uwe","family":"John","sequence":"additional","affiliation":[{"name":"Department Ecological Chemistry Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research Bremerhaven Germany"},{"name":"Helmholtz Institute for Functional Marine Biodiversity (HIFMB) Oldenburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4579-6607","authenticated-orcid":false,"given":"Ana","family":"Lokmer","sequence":"additional","affiliation":[{"name":"Department Coastal Ecology Wadden Sea Station Sylt Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research List\/Sylt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5735-4921","authenticated-orcid":false,"given":"Pieternella C.","family":"Luttikhuizen","sequence":"additional","affiliation":[{"name":"NIOZ Royal Netherlands Institute for Sea Research Department of Coastal Systems, and Utrecht University Den Burg The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2410-8898","authenticated-orcid":false,"given":"K. Mathias","family":"Wegner","sequence":"additional","affiliation":[{"name":"Department Coastal Ecology Wadden Sea Station Sylt Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research List\/Sylt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl140"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2007.09.008"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1420-9101.2011.02306.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2010.1211"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2012.00141"},{"key":"e_1_2_9_8_1","first-page":"861","article-title":"Chitinase in the mussel, Mytilus edulis (L.)","volume":"77","author":"Birkbeck T. H.","year":"1984","journal-title":"Comparative Biochemical Physiology"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.10.5322"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(92)90062-P"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2007.01.005"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2001.1945"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.68"},{"key":"e_1_2_9_15_1","first-page":"161","article-title":"\u00dcber Vorkommen und Metamorphose von Mytilicola intestinalis Steuer (Copepoda paras.) in der s\u00fcdlichen Nordsee","volume":"126","author":"Caspers H.","year":"1939","journal-title":"Zoologischer Anzeiger"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002905"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1242121"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2015.01.036"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2015.09.022"},{"key":"e_1_2_9_20_1","first-page":"R35","article-title":"Mytilicola intestinalis\u2014Serious pest or harmless commensal of mussels","volume":"75","author":"Davey J. T.","year":"1977","journal-title":"Parasitology"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06291"},{"key":"e_1_2_9_22_1","first-page":"344","article-title":"Zur Parasitologie der Miesmuschel (Mytilus edulis L., 1758)","volume":"22","author":"Dethlefsen V.","year":"1972","journal-title":"Berichte der Deutschen Wissenschaftlichen Kommission f\u00fcr Meeresforschung"},{"key":"e_1_2_9_23_1","unstructured":"Drinkwaard A. C.(1993).Het gevaar van de mosselparasiet voor de mosselkweek. Unpublished Report commissioned by Productschap voor Vis en Visproducten The Netherlands."},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1632"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6168"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2682680"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.zool.2016.05.012"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(91)90285-F"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-015-0481-x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123841"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092239"},{"key":"e_1_2_9_32_1","first-page":"103","article-title":"Immune\u2010related genes in gastropods and bivalves: A comparative overview","volume":"14","author":"Gerdol M.","year":"2017","journal-title":"Invertebrate Survival Journal"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2015.02.013"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seares.2015.12.003"},{"key":"e_1_2_9_35_1","article-title":"Trophic relationship between the invasive parasitic copepod Mytilicola orientalis and its native blue mussel (Mytilus edulis) host","author":"Goedknegt M. A.","year":"2017","journal-title":"Parasitology"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2010.10.002"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1883"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.zool.2016.06.010"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1161\/RES.0000000000000110"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.084"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/hdy.2017.50"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2004.49.2.0488"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0709994104"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3252"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms141121551"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu031"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-305X(99)00019-1"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1461-0248.2012.01742.x"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/v7031020"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2014.0295"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.2983\/035.035.0218"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.05.033"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.160"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108075"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(99)00088-6"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/icesjms\/38.1.6"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.112.192120"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12314"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148622"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep36695"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-806"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0159258"},{"key":"e_1_2_9_64_1","volume-title":"R: A language and environment for statistical computing","author":"R Core Team","year":"2017"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/eva.12109"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2016.03.013"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(94)90232-1"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2015.03.038"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12706"},{"key":"e_1_2_9_70_1","first-page":"263","article-title":"Molecular taxonomy in 2D: A novel ITS2 rRNA sequence\u2010structure approach guides the description of the oyster's subfamily Saccostreinae and the genus Magallana (Bivalvia: Ostreidae)","volume":"179","author":"Salvi D.","year":"2017","journal-title":"Zoological Journal of the Linnean Society"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2432"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1003113107"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00442"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1111\/eva.12370"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv351"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-8414-2_11"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2011.10.017"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn209"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-9-303"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20090049"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1099"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seares.2012.06.002"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00085"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2014.03.021"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxh328"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-69"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-13-78"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2852"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0129261"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.margen.2016.04.003"}],"container-title":["Molecular Ecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmec.14541","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mec.14541","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T15:48:47Z","timestamp":1695570527000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mec.14541"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3]]},"references-count":90,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,3]]}},"alternative-id":["10.1111\/mec.14541"],"URL":"https:\/\/doi.org\/10.1111\/mec.14541","archive":["Portico"],"relation":{},"ISSN":["0962-1083","1365-294X"],"issn-type":[{"value":"0962-1083","type":"print"},{"value":"1365-294X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3]]},"assertion":[{"value":"2017-07-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}