{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:15:48Z","timestamp":1787015748906,"version":"build-2736575974"},"reference-count":67,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,12,29]],"date-time":"2019-12-29T00:00:00Z","timestamp":1577577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011623","name":"Department of Biological Sciences, Purdue University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011623","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Variation in among\u2010family transcriptional responses to different environmental conditions can help to identify adaptive genetic variation, even prior to a selective event. Coupling differential gene expression with formal survival analyses allows for the disentanglement of treatment effects, required for understanding how individuals plastically respond to environmental stressors, from the adaptive genetic variation responsible for differential survival. We combined these two approaches to investigate responses to an emerging conservation issue, thiamine (vitamin B\n                    <jats:sub>1<\/jats:sub>\n                    ) deficiency, in a threatened population of Atlantic salmon (\n                    <jats:italic>Salmo salar<\/jats:italic>\n                    ). Thiamine is an essential vitamin that is increasingly limited in many ecosystems. In Lake Champlain, Atlantic salmon cannot acquire thiamine in sufficient quantities to support natural reproduction; fertilized eggs must be reared in hatcheries and treated with supplemental thiamine. We evaluated transcriptional responses (via RNA\u00a0sequencing) to thiamine treatment across families and found 3,616 genes differentially expressed between control (no supplemental thiamine) and treatment individuals. Fewer genes changed expression equally across families (i.e., additively) than exhibited genotype\u00a0\u00d7\u00a0environment interactions in response to thiamine. Differentially expressed genes were related to known physiological effects of thiamine deficiency, including oxidative stress, cardiovascular irregularities and neurological abnormalities. We also identified 1,446 putatively adaptive genes that were strongly associated with among\u2010family survival in the absence of thiamine treatment, many of which related to neurogenesis and visual perception. Our results highlight the utility of coupling RNA sequencing with formal survival analyses to identify candidate genes that underlie the among\u2010family variation in survival required for an adaptive response to natural selection.\n                  <\/jats:p>","DOI":"10.1111\/mec.15334","type":"journal-article","created":{"date-parts":[[2019,12,14]],"date-time":"2019-12-14T05:06:48Z","timestamp":1576300008000},"page":"1035-1049","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Among\u2010family variation in survival and gene expression uncovers adaptive genetic variation in a threatened fish"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7371-4002","authenticated-orcid":false,"given":"Avril M.","family":"Harder","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Purdue University West Lafayette IN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0176-1878","authenticated-orcid":false,"given":"Janna R.","family":"Willoughby","sequence":"additional","affiliation":[{"name":"Department of Forestry and Natural Resources Purdue University West Lafayette IN USA"},{"name":"School of Forestry and Wildlife Sciences Auburn University Auburn AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William R.","family":"Ardren","sequence":"additional","affiliation":[{"name":"U. 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