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Andean Condors (<jats:italic>Vultur gryphus<\/jats:italic>) are essential for ecosystem health and functioning, but their populations are globally near threatened and declining due to anthropogenic activities. During a microbiological and genomic surveillance study of critical priority antibiotic\u2010resistant pathogens, we identified pandemic lineages of multidrug\u2010resistant extended\u2010spectrum \u03b2\u2010lactamase (ESBL)\u2010producing <jats:italic>Escherichia coli<\/jats:italic> colonizing Andean Condors admitted at two wildlife rehabilitation centres in South America. Genomic analysis revealed the presence of genes encoding resistance to hospital and healthcare agents among international <jats:italic>E. coli<\/jats:italic> clones belonging to sequence types (STs) ST162, ST602, ST1196 and ST1485. In this regard, the resistome included genes conferring resistance to clinically important cephalosporins (i.e., CTX\u2010M\u201014, CTX\u2010M\u201055 and CTX\u2010M\u201065 ESBL genes), heavy metals (arsenic, mercury, lead, cadmium, copper, silver), pesticides (glyphosate) and domestic\/hospital disinfectants, suggesting a link with anthropogenic environmental pollution. On the other hand, the presence of virulence factors, including the <jats:italic>astA<\/jats:italic> gene associated with outbreak of childhood diarrhoea and extra\u2010intestinal disease in animals, was identified, whereas virulent behaviour was confirmed using the <jats:italic>Galleria mellonella<\/jats:italic> infection model. <jats:italic>E. coli<\/jats:italic> ST162, ST602, ST1196 and ST1485 have been previously identified in humans and food\u2010producing animals worldwide, indicating that a wide resistome could contribute to rapid adaptation and dissemination of these clones at the human\u2013animal\u2013environment interface. Therefore, these results highlight that Andean Condors have been colonized by critical priority pathogens, becoming potential environmental reservoirs and\/or vectors for dissemination of virulent and antimicrobial\u2010resistant bacteria and\/or their genes, in associated ecosystems and wildlife.<\/jats:p>","DOI":"10.1111\/mec.15455","type":"journal-article","created":{"date-parts":[[2020,4,26]],"date-time":"2020-04-26T12:50:20Z","timestamp":1587905420000},"page":"1919-1935","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Genomic data reveal international lineages of critical priority <i>Escherichia coli<\/i> harbouring wide resistome in Andean condors (<i>Vultur gryphus<\/i> Linnaeus, 1758)"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2845-4330","authenticated-orcid":false,"given":"Danny","family":"Fuentes\u2010Castillo","sequence":"first","affiliation":[{"name":"Department of Pathology School of Veterinary Medicine and Animal Sciences University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Esposito","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis Faculty of Pharmacy University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Biomedical Sciences University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gislaine","family":"Dalazen","sequence":"additional","affiliation":[{"name":"Department of Pathology School of Veterinary Medicine and Animal Sciences University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qu\u00e9zia","family":"Moura","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Biomedical Sciences University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna","family":"Fuga","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Biomedical Sciences University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herrison","family":"Fontana","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Biomedical Sciences University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Cerdeira","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Biomedical Sciences University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Dropa","sequence":"additional","affiliation":[{"name":"School of Public Health University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Rottmann","sequence":"additional","affiliation":[{"name":"Raptor Rehabilitation Centre Talagante Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Gonz\u00e1lez\u2010Acu\u00f1a","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences Faculty of Veterinary Sciences University of Concepci\u00f3n Chill\u00e1n Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 L.","family":"Cat\u00e3o\u2010Dias","sequence":"additional","affiliation":[{"name":"Department of Pathology School of Veterinary Medicine and Animal Sciences University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0161-5800","authenticated-orcid":false,"given":"Nilton","family":"Lincopan","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis Faculty of Pharmacy University of S\u00e3o Paulo Sao Paulo Brazil"},{"name":"Department of Microbiology Institute of Biomedical Sciences University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.39.1.315-335.1983"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.15101"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-015-0718-0"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2017.0387"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_9_7_1","article-title":"High\u2010level multidrug\u2010resistant Escherichia coli isolates from wild birds in a large urban environment","volume":"1","author":"Batalha de Jesus A. 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