{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T11:44:46Z","timestamp":1780487086599,"version":"3.54.1"},"reference-count":52,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T00:00:00Z","timestamp":1603324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001742","name":"United States-Israel Binational Science Foundation","doi-asserted-by":"publisher","award":["2015103"],"award-info":[{"award-number":["2015103"]}],"id":[{"id":"10.13039\/501100001742","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Vibrio cholerae<\/jats:italic>, the causative agent of cholera, is commonly isolated, along with other bacterial species, from chironomid insects (<jats:italic>Diptera: Chironomidae<\/jats:italic>). Nevertheless, its prevalence in the chironomid egg masses\u2019 microbiota is less than 0.5%. <jats:italic>V. cholerae<\/jats:italic> secretes haemagglutinin\/protease (HAP) that degrades the gelatinous matrix of chironomid egg masses and prevents hatching. Quorum sensing (QS) activates HAP production in response to accumulation of bacterial autoinducers (AIs). Our aim was to define the impact of chironomid microbiota on HAP production by <jats:italic>V.\u00a0cholerae<\/jats:italic>. To study QS signaling, we used <jats:italic>V.\u00a0cholerae<\/jats:italic> bioluminescence reporter strains (QS\u2010proficient O1 El\u2010Tor wild\u2010type and QS\u2010deficient mutants) and different bacterial species that we isolated from chironomid egg masses. These egg mass isolates, as well as a synthetic AI\u20102, caused an enhancement in <jats:italic>lux<\/jats:italic> expression by a <jats:italic>V.\u00a0cholerae<\/jats:italic> QS\u2010deficient mutant. The addition of the egg mass bacterial isolate supernatant to the QS\u2010deficient mutant also enhanced HAP production and egg mass degradation activities. Moreover, the <jats:italic>V.\u00a0cholerae<\/jats:italic> wild\u2010type strain was able to proliferate using egg masses as their sole carbon source, while the QS\u2010deficient was not. The results demonstrate that members of the chironomid bacterial consortium produce external chemical cues that, like AI\u20102, induce expression of the <jats:italic>hapA<\/jats:italic> gene in <jats:italic>V.\u00a0cholerae<\/jats:italic>. Understanding the interactions between <jats:italic>V.\u00a0cholerae<\/jats:italic> and the insects\u2019 microbiota may help uncover the interactions between this pathogen and the human gut microbiota.<\/jats:p>","DOI":"10.1111\/mec.15662","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T16:34:35Z","timestamp":1601570075000},"page":"1736-1746","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Quorum\u2010sensing signaling by chironomid egg masses\u2019 microbiota, affects haemagglutinin\/protease (HAP) production by <i>Vibrio cholerae<\/i>"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1798-2330","authenticated-orcid":false,"given":"Rotem","family":"Sela","sequence":"first","affiliation":[{"name":"Department of Evolutionary and Environmental Biology University of Haifa Haifa 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