{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:15:46Z","timestamp":1787015746712,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2022,6,19]],"date-time":"2022-06-19T00:00:00Z","timestamp":1655596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB1292, Z01"],"award-info":[{"award-number":["SFB1292, Z01"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["19\u2010382 07247S"],"award-info":[{"award-number":["19\u2010382 07247S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Few studies have examined tick proteomes, how they adapt to their environment, and their roles in the parasite\u2013host interactions that drive tick infestation and pathogen transmission. Here we used a proteomics approach to screen for biologically and immunologically relevant proteins acting at the tick\u2010host interface during tick feeding and, as proof of principle, measured host antibody responses to some of the discovered candidates. We used a label\u2010free quantitative proteomic workflow to study salivary proteomes of (i) wild\n                    <jats:italic>Ixodes ricinus<\/jats:italic>\n                    ticks fed on different hosts, (ii) wild or laboratory ticks fed on the same host, and (iii) adult ticks cofed with nymphs. Our results reveal high and stable expression of several protease inhibitors and other tick\u2010specific proteins under different feeding conditions. Most pathways functionally enriched in sialoproteomes were related to proteolysis, endopeptidase, and amine\u2010binding activities. The generated catalogue of tick salivary proteins enabled the selection of six candidate secreted immunogenic peptides for rabbit immunizations, three of which induced strong and durable antigen\u2010specific antibody responses in rabbits. Furthermore, rabbits exposed to ticks mounted immune responses against the candidate peptides\/proteins, confirming their expression at the tick\u2010vertebrate interface. Our approach provides insights into tick adaptation strategies to different feeding conditions and promising candidates for developing antitick vaccines or markers of exposure of vertebrate hosts to tick bites.\n                  <\/jats:p>","DOI":"10.1111\/mec.16561","type":"journal-article","created":{"date-parts":[[2022,6,4]],"date-time":"2022-06-04T03:43:59Z","timestamp":1654314239000},"page":"4162-4175","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Quantitative proteomics analysis reveals core and variable tick salivary proteins at the tick\u2010vertebrate host interface"],"prefix":"10.1111","volume":"31","author":[{"given":"Chaima","family":"Bensaoud","sequence":"first","affiliation":[{"name":"Institute of Parasitology, Biology Centre, Czech Academy of Sciences  Ceske Budejovice (Budweis) 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