{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T08:57:13Z","timestamp":1772787433392,"version":"3.50.1"},"reference-count":77,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2024,11,3]],"date-time":"2024-11-03T00:00:00Z","timestamp":1730592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","award":["J.0008.23"],"award-info":[{"award-number":["J.0008.23"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000233","name":"Genome British Columbia","doi-asserted-by":"publisher","award":["374PRO"],"award-info":[{"award-number":["374PRO"]}],"id":[{"id":"10.13039\/501100000233","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Hymenopteran queens are collectively highly fecund, often long\u2010lived individuals that undergo dramatic physiological changes after they mate and establish a nest. However, the degree to which these changes are conserved among species with different life histories is not well\u2010defined. We conducted a comparative proteomic study investigating differences between reproductive stages (virgin, mated and established queens) of <jats:italic>Apis mellifera<\/jats:italic>, <jats:italic>Bombus impatiens<\/jats:italic>, <jats:italic>B.\u2009terrestris<\/jats:italic> and <jats:italic>Lasius niger<\/jats:italic>. We analysed haemolymph for all species except <jats:italic>L.\u2009niger<\/jats:italic>, for which a whole\u2010body analysis was performed due to the small size of these queens. We identified conserved upregulation of proteins involved in anatomical and system development as queens transition to establishing a nest in all species except <jats:italic>B.\u2009terrestris<\/jats:italic>. We also identified conserved patterns of vitellogenin, vitellogenin receptor and immune\u2010responsive protein (IRP)30, all of which are proteins typically associated with oviposition. However, expression patterns of other immune proteins, heat\u2010shock proteins (HSPs), detoxification enzymes and antioxidant enzymes were more dissimilar, with some species exhibiting similar trends and co\u2010occurrence through reproductive stages, while others exhibited variable or opposite patterns. These conserved and unique profiles likely in part reflect similarities and differences in selective pressure on reproductive stages of each species and may indicate differing abilities to respond to emergent pathogens or environmental change.<\/jats:p>","DOI":"10.1111\/mec.17568","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T04:45:29Z","timestamp":1730695529000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Conserved and Unique Protein Expression Patterns Across Reproductive Stage Transitions in Social Hymenopteran Queens"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3523-6649","authenticated-orcid":false,"given":"Alison","family":"McAfee","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Michael Smith Laboratories University of British Columbia  Vancouver British Columbia Canada"},{"name":"Department of Applied Ecology North Carolina State University  Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baptiste","family":"Martinet","sequence":"additional","affiliation":[{"name":"Department of Evolutionary Biology &amp; Ecology Universit\u00e9 Libre de Bruxelles  Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly","family":"Przybyla","sequence":"additional","affiliation":[{"name":"Department of Zoology University of Mons  Mons Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e9licien","family":"Degueldre","sequence":"additional","affiliation":[{"name":"Department of Evolutionary Biology &amp; Ecology Universit\u00e9 Libre de Bruxelles  Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelley E.","family":"Hoover","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Lethbridge  Lethbridge Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serge","family":"Aron","sequence":"additional","affiliation":[{"name":"Department of Evolutionary Biology &amp; Ecology Universit\u00e9 Libre de Bruxelles  Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard J.","family":"Foster","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Michael Smith Laboratories University of British Columbia  Vancouver British Columbia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2011.09.006"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0333979100"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33065"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04698"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jeb.12964"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1570-7458.1998.00401.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2017.0380"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12330"},{"key":"e_1_2_11_10_1","first-page":"213","article-title":"Evolution and Function of the Insect Hexamerins","volume":"96","author":"Burmester T.","year":"2013","journal-title":"EJE"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1420-9101.2008.01664.x"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-66286-5"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.0c00776"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2017.09.009"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2018.2248"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00359-019-01362-0"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-019-6314-9"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2021.727124"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0638-x"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jisesa\/ieaa017"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2009.06.019"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.10730"},{"key":"e_1_2_11_23_1","unstructured":"Foster L.2023a. \u201cBombus impatiensReproduction and Immunity.\u201d Accession: MSV000091414.www.massive.ucsd.edu.https:\/\/doi.org\/10.25345\/C5TQ5RQ2K."},{"key":"e_1_2_11_24_1","unstructured":"Foster L.2023b. \u201cProteomic Investigation ofLasius nigerandBombus terrestrisQueen Fertility.\u201d Accession: MSV000092460.www.massive.ucsd.edu.https:\/\/doi.org\/10.25345\/C58G8FT6W."},{"key":"e_1_2_11_25_1","unstructured":"Foster L.2024. \u201cHemolymph of Queen Honey Bees at Different Reproductive Stages.\u201d Accession: MSV000094277.www.massive.ucsd.edu.https:\/\/doi.org\/10.25345\/C5MC8RT0S."},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.2c00145"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13592-017-0546-y"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.795669"},{"key":"e_1_2_11_29_1","volume-title":"Bumblebee Economics","author":"Heinrich B.","year":"2004"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2023.107963"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2016.00574"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5967.2008.00142.x"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2009.00965.x"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-232"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12862-016-0867-x"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u20106\u2010269"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1080\/00379271.2013.774949"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.45009"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects13100862"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.14256"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1750-7"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0118591"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/1365-2435.13738"},{"key":"e_1_2_11_44_1","first-page":"4","volume-title":"Hymenoptera of the World: An Identification Guide to Families","author":"Mason W. R.","year":"1993"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2023.2463"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010020\u201001586\u2010w"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2024.110906"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03092-7"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010020\u201006992\u20102"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12020245"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12758"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu171"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13592-015-0418-2"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2015.2409"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.46.1.601"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(03)00021-3"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2021.748405"},{"key":"e_1_2_11_58_1","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2023"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0244648"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac026117i"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000980"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep10982"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2016.04.013"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005015"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evw014"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-010715-023924"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2016.06.001"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389203720666191111113726"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-74456-4"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2021.145446"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33207"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2021.116566"},{"key":"e_1_2_11_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2021.112105"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49025-z"},{"key":"e_1_2_11_76_1","volume-title":"The Biology of the Honey Bee","author":"Winston M. L.","year":"1991"},{"key":"e_1_2_11_77_1","first-page":"93","article-title":"Chilling Temperature and Humidity to Break Diapause of the Bumblebee Queen Bombus terrestris","volume":"20","author":"Yoon H.\u2010J.","year":"2010","journal-title":"International Journal of Industrial Entomology"},{"key":"e_1_2_11_78_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.7736"}],"container-title":["Molecular Ecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mec.17568","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T08:59:41Z","timestamp":1732611581000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mec.17568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,3]]},"references-count":77,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1111\/mec.17568"],"URL":"https:\/\/doi.org\/10.1111\/mec.17568","archive":["Portico"],"relation":{},"ISSN":["0962-1083","1365-294X"],"issn-type":[{"value":"0962-1083","type":"print"},{"value":"1365-294X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,3]]},"assertion":[{"value":"2024-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e17568"}}