{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T19:06:06Z","timestamp":1792177566017,"version":"4.4.0"},"reference-count":84,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100023581","name":"National Science Foundation Graduate Research Fellowship Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100023581","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1923743"],"award-info":[{"award-number":["1923743"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["NNCI\u20102025608"],"award-info":[{"award-number":["NNCI\u20102025608"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Corals residing in habitats that experience high\u2010frequency seawater pCO\n 2<\/jats:sub>\n variability may possess an enhanced capacity to cope with ocean acidification, yet we lack a clear understanding of the molecular toolkit enabling acclimatisation to environmental extremes or how life\u2010long exposure to pCO\n 2<\/jats:sub>\n variability influences biomineralisation. Here, we examined the gene expression responses and micro\u2010skeletal characteristics of\n \n Pocillopora damicornis<\/jats:italic>\n <\/jats:styled-content>\n originating from the reef flat and reef slope of Heron Island, southern Great Barrier Reef. The reef flat and reef slope had similar mean seawater pCO\n 2<\/jats:sub>\n , but the reef flat experienced twice the mean daily pCO\n 2<\/jats:sub>\n amplitude (range of 797 v. 399 \u03bcatm day\n \u22121<\/jats:sup>\n , respectively). A controlled mesocosm experiment was conducted over 8\u2009weeks, exposing\n \n P. damicornis<\/jats:italic>\n <\/jats:styled-content>\n from the reef slope and reef flat to stable (218\u2009\u00b1\u20099) or variable (911\u2009\u00b1\u200931) diel pCO\n 2<\/jats:sub>\n fluctuations (\u03bcatm; mean\u2009\u00b1\u2009SE). At the end of the exposure,\n \n P. damicornis<\/jats:italic>\n <\/jats:styled-content>\n originating from the reef flat demonstrated frontloading of 25% of the expressed genes regardless of treatment conditions, suggesting constitutive upregulation. This included higher expression of critical biomineralisation\u2010related genes such as carbonic anhydrases, skeletal organic matrix proteins, and bicarbonate transporters. The observed frontloading corresponded with a 40% increase of the fastest deposited areas of the skeleton in reef flat corals grown under non\u2010native, stable pCO\n 2<\/jats:sub>\n conditions compared to reef slope conspecifics, suggesting a compensatory response that stems from acclimatisation to environmental extremes and\/or relief from stressful pCO\n 2<\/jats:sub>\n fluctuations. Under escalating ocean warming and acidification, corals acclimated to environmental variability warrant focused investigation and represent ideal candidates for active interventions to build reef resilience while societies adopt strict policies to limit climate change.\n <\/jats:p>","DOI":"10.1111\/mec.17603","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T04:12:40Z","timestamp":1732767160000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Extreme Environmental Variability Induces Frontloading of Coral Biomineralisation Genes to Maintain Calcification Under\n \n pCO\n 2<\/sub>\n <\/scp>\n Variability"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5235-5928","authenticated-orcid":false,"given":"Kristen T.","family":"Brown","sequence":"first","affiliation":[{"name":"Department of Biology University of Pennsylvania Philadelphia Pennsylvania USA"},{"name":"School of Biological Sciences University of Queensland St. Lucia Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4102-1492","authenticated-orcid":false,"given":"Zoe","family":"Dellaert","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Rhode Island Kingston Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6157-2502","authenticated-orcid":false,"given":"Marcelina P.","family":"Martynek","sequence":"additional","affiliation":[{"name":"Department of Biology University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1322-1220","authenticated-orcid":false,"given":"Julia","family":"Durian","sequence":"additional","affiliation":[{"name":"Department of Biology University of Pennsylvania Philadelphia Pennsylvania USA"},{"name":"Oberlin College Oberlin Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7298-290X","authenticated-orcid":false,"given":"Tali","family":"Mass","sequence":"additional","affiliation":[{"name":"Department of Marine Biology, Leon H. Charney School of Marine Sciences University of Haifa Haifa Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2322-3269","authenticated-orcid":false,"given":"Hollie M.","family":"Putnam","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Rhode Island Kingston Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7371-4870","authenticated-orcid":false,"given":"Katie L.","family":"Barott","sequence":"additional","affiliation":[{"name":"Department of Biology University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2486.2010.02364.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2025435118"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00052.2015"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.02.115"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1210224110"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2014.10.044"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10126-011-9363-x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00338\u2010024\u201002484\u2010x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2022.0941"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ecy.3324"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps13073"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2023.168552"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps10690"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2018.1168"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2015265118"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-34459-8"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00428135"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43247-020-00054-x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1301419110"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/gcb.14912"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw354"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aao1118"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.5670\/oceanog.2009.95"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4794"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crvi.2007.12.003"},{"key":"e_1_2_10_28_1","volume-title":"Package \u201ccar\u201d","author":"Fox J.","year":"2012"},{"key":"e_1_2_10_29_1","unstructured":"Gentleman R. 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J.Bass A.Dabney andD.Robinson.2023.\u201cqvalue: Q\u2010value estimation for false discovery rate control.\u201d(Version version 2.32.0) [R package].http:\/\/github.com\/jdstorey\/qvalue."},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2015.02.018"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/gcb.15372"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2216144120"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-021-01007-8"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1422301112"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1216153110"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0030-1299.2004.13255.x"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam6371"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1111\/gcb.15148"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1111\/gcb.14764"},{"key":"e_1_2_10_83_1","first-page":"1","article-title":"goseq: Gene Ontology Testing for RNA\u2010Seq Datasets","volume":"8","author":"Young M. 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