{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:22:30Z","timestamp":1787498550444,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T00:00:00Z","timestamp":1733788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009792","name":"Kennesaw State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009792","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Alternative reproductive tactics are discrete, intrasexual differences in reproductive behaviour within a population. In some cases, these complex phenotypes are determined by autosomal supergenes or sex chromosomes\u2014both of which exhibit reduced recombination and thus enable the linked inheritance of co\u2010adapted alleles from multiple loci. Most alternative reproductive tactics in amphibians are plastic (and reversible), environmentally determined and lacking morphological differentiation, but a striking exception is found in the two\u2010lined salamander (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Eurycea bislineata<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) species complex. In some populations, two distinct male phenotypes coexist: \u2018searching\u2019 males have mental glands, protruding premaxillary teeth and elongate cirri used in terrestrial courtship, while \u2018guarding\u2019 males lack these traits and instead have hypertrophied jaw musculature used in mate guarding at aquatic nesting sites. These tactics differ in many morphological and behavioural phenotypes, but their proximate cause has not yet been described. Here, we generated genome\u2010wide SNP data from &gt;\u2009130\n                    <jats:italic>Eurycea<\/jats:italic>\n                    cf.\n                    <jats:italic>wilderae<\/jats:italic>\n                    collected from Highlands Biological Station. We provide evidence for an XY sex determination and for a Y\u2010linked polymorphism underlying alternative reproductive tactics in this population. We then develop and validate a PCR\u2010based genotyping assay and apply it to characterise the sex ratio and relative frequency of male tactics from a sample of larvae. Our results add to the growing body of literature exploring the importance of supergenes and sex chromosomes in complex intraspecific polymorphisms, and we highlight opportunities for future work to continue exploring the genomic architecture of these traits.\n                  <\/jats:p>","DOI":"10.1111\/mec.17621","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T06:55:45Z","timestamp":1733813745000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Genetic Polymorphism Underlying Alternative Reproductive Tactics in\n                    <i>Eurycea<\/i>\n                    Salamanders"],"prefix":"10.1111","volume":"34","author":[{"given":"Yatin","family":"Kalki","sequence":"first","affiliation":[{"name":"Department of Ecology, Evolution, and Organismal Biology Kennesaw State University  Kennesaw Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3818-5146","authenticated-orcid":false,"given":"Todd W.","family":"Pierson","sequence":"additional","affiliation":[{"name":"Department of Ecology, Evolution, and Organismal Biology Kennesaw State University  Kennesaw Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,10]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Arnold S. J.1972.\u201cThe Evolution of Courtship Behavior in Salamanders.\u201dPhD Dissertation University of Michigan."},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/24.2.309"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001899"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.7724"},{"key":"e_1_2_9_6_1","first-page":"2022","article-title":"Sequencing and Chromosome\u2010Scale Assembly of the Giant \n                     Pleurodeles waltl\n                   Genome","author":"Brown T.","year":"2022","journal-title":"BioRxiv"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2307\/1445917"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/eva.12291"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2021.0202"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz966"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1655\/Herpetologica-D-23-00036"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1890\/0012-9658(2006)87[1445:SOEFDD]2.0.CO;2"},{"key":"e_1_2_9_13_1","volume-title":"Genetic Polymorphism","author":"Ford E. B.","year":"1965"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.42.110807.091714"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-5347(96)81050-0"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evab057"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syaa034"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1317165111"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3443"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3430"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.hdy.6800386"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-022-00524-2"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000485270"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3241"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25458"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/evo.14321"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-88178-7"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511542602"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.2009.00837.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2020.0094"},{"key":"e_1_2_9_31_1","first-page":"247","article-title":"Divergent Reproductive Phenologies in Male Eurycea With Alternative Reproductive Tactics","volume":"50","author":"Pierson T. W.","year":"2019","journal-title":"Herpetological Review"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1086\/702251"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1655\/Herpetologica-D-23-00046"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syad064"},{"key":"e_1_2_9_35_1","unstructured":"R Core Team.2023.\u201cR: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing.\u201dhttps:\/\/www.R\u2010project.org."},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1643\/h2020010"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac975"},{"key":"e_1_2_9_38_1","first-page":"1","article-title":"Easy\u2010To\u2010Use R Functions to Separate Reduced\u2010Representation Genomic Datasets Into Sex\u2010Linked and Autosomal Loci, and Conduct Sex Assignment","volume":"00","author":"Robledo\u2010Ruiz D. 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