{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T03:59:13Z","timestamp":1782100753122,"version":"3.54.5"},"reference-count":135,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T00:00:00Z","timestamp":1737072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100020658","name":"NOAA Sea Grant","doi-asserted-by":"publisher","award":["NA22OAR4170104"],"award-info":[{"award-number":["NA22OAR4170104"]}],"id":[{"id":"10.13039\/100020658","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Anthropogenic carbon dioxide emissions have been increasing rapidly in recent years, driving pH and oxygen levels to record low concentrations in the oceans. Eastern boundary upwelling systems such as the California Current System (CCS) experience exacerbated ocean acidification and hypoxia (OAH) due to the physical and chemical properties of the transported deeper waters. Research efforts have significantly increased in recent years to investigate the deleterious effects of climate change on marine species, but have not focused on the impacts of simultaneous OAH stressor exposure. Additionally, few studies have explored the physiological impacts of these environmental stressors on the earliest life stages, which are more vulnerable and represent natural population bottlenecks in organismal life cycles. The physiological response of the ecologically and commercially important red sea urchin (<jats:italic>Mesocentrotus franciscanus<\/jats:italic>) was assessed by exposing larvae to a variety of OAH conditions, mimicking the range of ecologically relevant conditions encountered currently and in the near future along the CCS. Skeleton dissolution, larval development, and gene expression show a response with clearly delineated thresholds that were related to OAH severity. Skeletal dissolution and the induction of <jats:italic>Acid\u2010sensing Ion Channel 1A<\/jats:italic> at pH\u20097.94\/5.70 DO mg\/L provide particularly sensitive markers of OAH, with dramatic shifts in larval morphology and gene expression detected at the pH\/DO transition of 7.71\/3.71\u20137.27\/2.72\u2009mg\/L. Experimental simulations that describe physiological thresholds and establish molecular markers of OAH exposure will provide fishery management with the tools to predict patterns of larval recruitment and forecast population dynamics.<\/jats:p>","DOI":"10.1111\/mec.17658","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T11:43:46Z","timestamp":1737114226000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Single\u2010Larva <scp>RNA<\/scp> Sequencing Reveals That Red Sea Urchin Larvae Are Vulnerable to Co\u2010Occurring Ocean Acidification and Hypoxia"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9942-7417","authenticated-orcid":false,"given":"Tina","family":"Nguyen","sequence":"first","affiliation":[{"name":"Marine and Environmental Biology University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg","family":"Pelletier","sequence":"additional","affiliation":[{"name":"Washington Department of Ecology  Olympia Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Bednar\u0161ek","sequence":"additional","affiliation":[{"name":"Cooperative Institute for Marine Resources Studies, Hatfield Marine Science Center Oregon State University  Corvallis Oregon USA"},{"name":"Department of Environmental Sciences Jozef Stefan Institute  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Gracey","sequence":"additional","affiliation":[{"name":"Marine and Environmental Biology University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,17]]},"reference":[{"issue":"40","key":"e_1_2_10_2_1","first-page":"343","article-title":"Status and Management of World Sea Urchin Fisheries","volume":"40","author":"Andrew N.","year":"2002","journal-title":"Oceanography and Marine Biology"},{"key":"e_1_2_10_3_1","unstructured":"Andrews S.2019. \u201cFastQC: A Quality Control Tool for High Throughput Sequence Data.\u201dhttps:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2021.02.014"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ecs2.3015"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.2c00010"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.136610"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.5670\/oceanog.2023.210"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2014.0123"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2021.671497"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102486.2012.02668.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1997.0066"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010marine\u2010121211\u2010172411"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/bf02804914"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1029\/2008GL034185"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioadv\/vbad123"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440\u2010169X.1995.00008.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.12.063"},{"key":"e_1_2_10_19_1","unstructured":"Bushnell B.2015. \u201cBBMap.\u201dhttps:\/\/sourceforge.net\/projects\/bbmap\/."},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2012.0439"},{"key":"e_1_2_10_21_1","first-page":"251","volume-title":"Evolutionary Ecology of Marine Invertebrate Larvae","author":"Byrne M.","year":"2018"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fishres.2016.03.001"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1149016"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.02.037"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1029\/2020GL089553"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps11852"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1332"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.044149"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1419141111"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps11142"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1029\/2021JC018159"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.15664"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/icae096"},{"key":"e_1_2_10_34_1","volume-title":"Guide to Best Practices for Ocean CO2 Measurments","author":"Dickson A.","year":"2007"},{"key":"e_1_2_10_35_1","unstructured":"Dickson A. G.1990.\u201cThe Oceanic Carbon Dioxide System: Planning for Quality Data.\u201d"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2021.782583"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2019.113850"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/icesjms\/fsr123"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/gcb.12276"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1086\/BBLv226n3p223"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1.10.1280"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12188"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.14038"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.5194\/essd\u201010\u20101367\u20102018"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1029\/2023GB007843"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecss.2016.08.043"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1029\/2023JC019892"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.5520080102"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1086\/696830"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1029\/2018JC013934"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.2850"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/icaa072"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-016-1683-5"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2015.0976"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083648"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1883"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1021\/es501564q"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9112379"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.647482"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010023\u201001767\u2010y"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ecolsys.110308.120227"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2020.1506"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00007.2023"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.36600"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467\u20102979.2005.00189.x"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1093\/conphys\/coaa013"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2004.11.027"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/gcb.13833"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.5194\/essd\u201016\u2010219\u20102024"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2018856118"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076\u20106879(87)39098\u20106"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1525\/elementa.198"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2016.0802"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps274161"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.195859"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2019.04.018"},{"key":"e_1_2_10_78_1","unstructured":"Leighton D. L. L. G.Jones andW. J.North.1966.\u201cEcological Relationships Between Giant Kelp and Sea Urchins In Southern California.\u201dInProceedings of the 5th International Seaweed Symposium Halifax Amsterdam: Elsevier.https:\/\/doi.org\/10.1016\/b978\u20100\u201008\u2010011841\u20103.50023\u20109."},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.02.021"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.07.047"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010014\u20100550\u20108"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304\u20104203(00)00022\u20100"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1806614115"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2020.00670"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1175\/1520\u20100485(2003)33<753:ESADOT>2.0.CO;2"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00227\u2010012\u20101954\u20101"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps148083"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-35088-7_14"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1180"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpol.2007.04.004"},{"key":"e_1_2_10_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.735618"},{"key":"e_1_2_10_92_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20133278"},{"key":"e_1_2_10_93_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps08346"},{"key":"e_1_2_10_94_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2013.0155"},{"key":"e_1_2_10_95_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1416967112"},{"key":"e_1_2_10_96_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4197"},{"issue":"3","key":"e_1_2_10_97_1","first-page":"385","article-title":"Distribution of Echinoderm Larval Populations in the Geostrophic Frontal Jet of the Eastern Alboran Sea","volume":"19","author":"Pedrotti M. L.","year":"1996","journal-title":"Oceanologica Acta"},{"key":"e_1_2_10_98_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.10.037"},{"key":"e_1_2_10_99_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.240705"},{"key":"e_1_2_10_100_1","doi-asserted-by":"publisher","DOI":"10.2307\/2269573"},{"key":"e_1_2_10_101_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044\u20108486(00)00316\u20101"},{"key":"e_1_2_10_102_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M507044200"},{"key":"e_1_2_10_103_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1102289"},{"key":"e_1_2_10_104_1","volume-title":"RStudio: Integrated Development for R","author":"Posit Team","year":"2023"},{"key":"e_1_2_10_105_1","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team (2022)","year":"2020"},{"key":"e_1_2_10_106_1","doi-asserted-by":"publisher","DOI":"10.5670\/oceanog.2014.21"},{"key":"e_1_2_10_107_1","unstructured":"Rakaj A. L.Morroni G.Flori et\u00a0al. n.d. \u201cTowards Automatization in Morphometric Analysis of Sea Urchin Larvae for 2 Ecotoxicological Applications and Near\u2010Future Scenarios Simulation 34.\u201dhttps:\/\/ssrn.com\/abstract=4788439."},{"key":"e_1_2_10_108_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.22754"},{"key":"e_1_2_10_109_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.7198205"},{"key":"e_1_2_10_110_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978\u20100\u201012\u2010819570\u20103.00032\u20109"},{"key":"e_1_2_10_111_1","doi-asserted-by":"publisher","DOI":"10.1038\/73432"},{"key":"e_1_2_10_112_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053118"},{"key":"e_1_2_10_113_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0021\u20109258(17)31580\u20106"},{"key":"e_1_2_10_114_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1109243109"},{"key":"e_1_2_10_115_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ocemod.2004.08.002"},{"key":"e_1_2_10_116_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011372"},{"key":"e_1_2_10_117_1","doi-asserted-by":"publisher","DOI":"10.5194\/bg\u201018\u20102871\u20102021"},{"key":"e_1_2_10_118_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps11908"},{"key":"e_1_2_10_119_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2011.06.023"},{"key":"e_1_2_10_120_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506580102"},{"key":"e_1_2_10_121_1","first-page":"107","article-title":"Revised Procedure for Staining and Clearing Small Fishes and Other Vertebrates for Bone and Cartilage Study","volume":"9","author":"Taylor W. R.","year":"1985","journal-title":"Cybium"},{"key":"e_1_2_10_122_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0196864"},{"key":"e_1_2_10_123_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102818.2005.01486.x"},{"key":"e_1_2_10_124_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.032540"},{"key":"e_1_2_10_125_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803833105"},{"key":"e_1_2_10_126_1","doi-asserted-by":"publisher","DOI":"10.1080\/15366367.2019.1565254"},{"key":"e_1_2_10_127_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.08.064"},{"key":"e_1_2_10_128_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001404"},{"key":"e_1_2_10_129_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8793"},{"key":"e_1_2_10_130_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20045"},{"key":"e_1_2_10_131_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2008.01.036"},{"key":"e_1_2_10_132_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1995.40.2.0316"},{"key":"e_1_2_10_133_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.margen.2019.05.007"},{"key":"e_1_2_10_134_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00227\u2010019\u20103633\u2010y"},{"key":"e_1_2_10_135_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0025\u2010326X(02)00061\u20109"},{"key":"e_1_2_10_136_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201021038\u20101"}],"container-title":["Molecular Ecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mec.17658","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T04:43:22Z","timestamp":1740545002000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mec.17658"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,17]]},"references-count":135,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1111\/mec.17658"],"URL":"https:\/\/doi.org\/10.1111\/mec.17658","archive":["Portico"],"relation":{},"ISSN":["0962-1083","1365-294X"],"issn-type":[{"value":"0962-1083","type":"print"},{"value":"1365-294X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,17]]},"assertion":[{"value":"2024-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e17658"}}