{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:42:44Z","timestamp":1791909764407,"version":"4.3.4"},"reference-count":111,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T00:00:00Z","timestamp":1738540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T00:00:00Z","timestamp":1738540800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270441"],"award-info":[{"award-number":["32270441"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cetaceans show remarkable diversity in diving capability, implying a range of adaptive strategies to hazards such as hydrostatic pressure and oxidative stress, but few studies have considered the evolution of extreme diving. Here, we first examined the relationship between morphological and physiological factors and diving capability and then considered the molecular evolution of candidate deep\u2010sea diving traits in a genomic dataset of cetaceans. Our dataset included six super\u2010divers, sperm whales (families Physeteridae and Kogiidae) and beaked whales (Ziphiidae), species that can dive deeper than 1000\u2009m for about an hour or longer. We found a positive association between diving capability and oxygen\u2010linked globins, and super\u2010diver myoglobin (\n                    <jats:italic>MB<\/jats:italic>\n                    ) is under positive selection and harbours a reported functional amino acid change. Blubber thickness was positively associated, likely to provide thermal insulation and hydrostatic pressure resistance. Super\u2010divers have gene changes that may contribute to differences in the composition of outer blubber neutral lipids (triacylglycerols and wax esters), fatty acids and cholesterol. Total lung capacity relative to body mass showed a negative association, ostensibly to limit gas bubbles that can cause decompression sickness. A functional assay suggests that an\n                    <jats:italic>ATP8B1<\/jats:italic>\n                    amino acid substitution may reduce lung injury in super\u2010divers. Super\u2010diver\n                    <jats:italic>XDH<\/jats:italic>\n                    has two unique amino acids and a decreased ability to produce uric acid under hypoxia when its ROS\u2010generating XO isoform is prevalent, suggesting that it reduces cell damage from oxidative stress and uric acid accumulation in species with prolonged dives. Our study deepens the understanding of how deep\u2010sea diving emerged in the cetacean lineage.\n                  <\/jats:p>","DOI":"10.1111\/mec.17678","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T06:34:36Z","timestamp":1738564476000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Comparative Genomics Uncovers Molecular Adaptations for Cetacean Deep\u2010Sea Diving"],"prefix":"10.1111","volume":"34","author":[{"given":"Fan","family":"Zhang","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory for the Biodiversity Conservation and Sustainable Utilization in the Middle and Lower Reaches of the Yangtze River Basin College of Life Sciences, Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for the Biodiversity Conservation and Sustainable Utilization in the Middle and Lower Reaches of the Yangtze River Basin College of Life Sciences, Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Dong","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for the Biodiversity Conservation and Sustainable Utilization in the Middle and Lower Reaches of the Yangtze River Basin College of Life Sciences, Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6285-6937","authenticated-orcid":false,"given":"Guang","family":"Yang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for the Biodiversity Conservation and Sustainable Utilization in the Middle and Lower Reaches of the Yangtze River Basin College of Life Sciences, Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inge","family":"Seim","sequence":"additional","affiliation":[{"name":"Marine Mammal and Marine Bioacoustics Laboratory Institute of Deep\u2010Sea Science and Engineering, Chinese Academy of Sciences  Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4564-1886","authenticated-orcid":false,"given":"Ran","family":"Tian","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for the Biodiversity Conservation and Sustainable Utilization in the Middle and Lower Reaches of the Yangtze River Basin College of Life Sciences, Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmsc.2001.1159"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2021.11.008"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01199"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-020-1101-3"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3106\/mammalstudy.28.103"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/5584_2021_634"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00080.2016"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3527579"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2020.110843"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-397002-2.00010-7"},{"key":"e_1_2_10_13_1","volume-title":"Behavior and Ecology of Pacific White\u2010Sided Dolphins (Lagenorhynchus obliquidens) in Monterey Bay, California","author":"Black N. A.","year":"1994"},{"key":"e_1_2_10_14_1","first-page":"57","volume-title":"Handbook of Marine Mammals","author":"Brownell R. L.","year":"1999"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp348"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-23-0391"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026334"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2118656119"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.2307\/1943075"},{"key":"e_1_2_10_20_1","first-page":"82","article-title":"Pymol: An Open\u2010Source Molecular Graphics Tool","volume":"40","author":"DeLano W. L.","year":"2002","journal-title":"CCP4 Newsletter. Protein Crystallography"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3992247"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1502549112"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-01916-y"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2118145119"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2016.10.020"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1993.1204"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1832-y"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M212780200"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP091095"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2008.09.013"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.126870"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2020.555509"},{"key":"e_1_2_10_33_1","first-page":"471","article-title":"Maximum\u2010Likelihood Estimation of Evolutionary Trees From Continuous Characters","volume":"25","author":"Felsenstein J.","year":"1973","journal-title":"American Journal of Human Genetics"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsr.2021.103492"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13023-015-0290-1"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1344"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2006.04.012"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-006-0083-1"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41559-017-0336-y"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2018.10.007"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-018-2779-y"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101742"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx302"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.166033"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2005.01.004"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1997.tb01457.x"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.148338"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2004.2964"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.054"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102920"},{"key":"e_1_2_10_51_1","first-page":"57","article-title":"Abundance Decline in the Finless Porpoise Population in the Inland Sea of Japan","volume":"50","author":"Kasuya T.","year":"2002","journal-title":"Raffles Bulletin of Zoology"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.12.008"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1043"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.161471"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260590"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abn3315"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0391-5"},{"key":"e_1_2_10_58_1","first-page":"1","article-title":"Body Composition of the Sperm Whale, Physeter catodon, with Special Reference to the Possible Function of Fat Depots","volume":"12","author":"Lockyer C.","year":"1991","journal-title":"Rit Fiskideildar"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10279-w"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-646-7_10"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-7692.2006.00042.x"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-014-2282-1"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2022.01.003"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2013.10.021"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2007.02.012"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1234192"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci9080424"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2022.101157"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0706.2003.12010.x"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/39.2.434"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.120.14481"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg509"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(00)00182-3"},{"issue":"2","key":"e_1_2_10_74_1","first-page":"1","article-title":"The Caper Package: Comparative Analysis of Phylogenetics and Evolution in R","volume":"5","author":"Orme D.","year":"2013","journal-title":"R Package Version"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00841"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12862-019-1525-x"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0207395"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.639377"},{"key":"e_1_2_10_79_1","first-page":"D845","article-title":"The DisGeNET Knowledge Platform for Disease Genomics: 2019 Update","volume":"48","author":"Pi\u00f1ero J.","year":"2020","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.10823"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.20192"},{"key":"e_1_2_10_82_1","first-page":"341","volume-title":"Life in Extreme Environments","author":"Pradillon F.","year":"2007"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8040319"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy159"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2213"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adg6568"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0226206"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1501694"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.40686"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201700947R"},{"key":"e_1_2_10_91_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00032.2017"},{"key":"e_1_2_10_92_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsr.2020.103285"},{"key":"e_1_2_10_93_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep15909"},{"key":"e_1_2_10_94_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0807919106"},{"key":"e_1_2_10_95_1","doi-asserted-by":"publisher","DOI":"10.1086\/589108"},{"key":"e_1_2_10_96_1","first-page":"110","article-title":"FasParser: A Package for Manipulating Sequence Data","volume":"38","author":"Sun Y. B.","year":"2017","journal-title":"Zoological Research"},{"key":"e_1_2_10_97_1","doi-asserted-by":"publisher","DOI":"10.1111\/1749-4877.12610"},{"key":"e_1_2_10_98_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2020.158842"},{"key":"e_1_2_10_99_1","doi-asserted-by":"publisher","DOI":"10.24272\/j.issn.2095-8137.2021.420"},{"key":"e_1_2_10_100_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2017.00205"},{"key":"e_1_2_10_101_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41559-019-0864-8"},{"key":"e_1_2_10_102_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.75796"},{"key":"e_1_2_10_103_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2656.2006.01101.x"},{"key":"e_1_2_10_104_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12020283"},{"key":"e_1_2_10_105_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00017.2015"},{"key":"e_1_2_10_106_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1062"},{"key":"e_1_2_10_107_1","volume-title":"Encyclopedia of Marine Mammals","author":"Wursig B.","year":"2009"},{"key":"e_1_2_10_108_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm088"},{"key":"e_1_2_10_109_1","article-title":"Comparative Analyses of Aging\u2010Related Genes in Long\u2010Lived Mammals Provide Insights Into Natural Longevity","volume":"2","author":"Yu Z.","year":"2021","journal-title":"Innovations"},{"key":"e_1_2_10_110_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)00905-X"},{"key":"e_1_2_10_111_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002399910007"},{"key":"e_1_2_10_112_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adh0474"}],"container-title":["Molecular Ecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mec.17678","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mec.17678","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mec.17678","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T07:09:10Z","timestamp":1771312150000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mec.17678"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,3]]},"references-count":111,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1111\/mec.17678"],"URL":"https:\/\/doi.org\/10.1111\/mec.17678","archive":["Portico"],"relation":{},"ISSN":["0962-1083","1365-294X"],"issn-type":[{"value":"0962-1083","type":"print"},{"value":"1365-294X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,3]]},"assertion":[{"value":"2024-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e17678"}}