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Theoretical models and controlled crosses support the \u2018triploid bridge\u2019 hypothesis, proposing that hybrids of intermediate ploidy facilitate gene flow. However, comprehensive evidence combining experimental and genetic data from natural mixed\u2010ploidy species is missing. Here, we investigated the rates and directionality of gene flow within a diploid\u2010autotetraploid contact zone of <jats:italic>Cardamine amara<\/jats:italic>, a species with abundant natural triploids. We cytotyped over 400 individuals in the field, conducted reciprocal interploidy crosses, and inferred gene flow based on genome\u2010wide sequencing of 84 individuals. Triploids represent a conspicuous entity in mixed\u2010ploidy populations (5%), yet only part of them arose through interploidy hybridisation. Despite being rarely formed, triploid hybrids can backcross with their parental cytotypes, producing viable offspring that are often euploid (in 42% of cases). In correspondence, D\u2010statistics and coalescent simulations documented a significant genome\u2010wide signal of bidirectional gene flow in sympatric but not allopatric populations. Triploids, though rare, thus seem to play a key role in overcoming polyploidy\u2010related reproductive barriers in <jats:styled-content style=\"fixed-case\"><jats:italic>C. amara<\/jats:italic><\/jats:styled-content>. In sum, we present integrative evidence for interploidy gene flow mediated by a triploid bridge in natural populations.<\/jats:p>","DOI":"10.1111\/mec.17702","type":"journal-article","created":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T05:54:21Z","timestamp":1740549261000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Overcoming Ploidy Barriers: The Role of Triploid Bridges in the Genetic Introgression of <i>Cardamine amara<\/i>"],"prefix":"10.1111","volume":"34","author":[{"given":"P.","family":"Bartoli\u0107","sequence":"first","affiliation":[{"name":"Department of Botany, Faculty of Science Charles University in Prague  Prague Czechia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Voltrov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Science Charles University in Prague  Prague 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Technology Masaryk University  Brno Czechia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Padilla Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Bot\u00e1nica y Fisiolog\u00eda Vegetal University of Salamanca  Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7658-0844","authenticated-orcid":false,"given":"K.","family":"Marhold","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Science Charles University in Prague  Prague Czechia"},{"name":"Institute of Botany Plant Science and Biodiversity Centre, Slovak Academy of Sciences  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8793-7992","authenticated-orcid":false,"given":"F.","family":"Kol\u00e1\u0159","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Science Charles University in Prague 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