{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T01:53:21Z","timestamp":1781574801677,"version":"3.54.5"},"reference-count":103,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1754331"],"award-info":[{"award-number":["1754331"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2148203"],"award-info":[{"award-number":["2148203"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001024","name":"Roy J. Carver Charitable Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2022\/14347\u20101"],"award-info":[{"award-number":["2022\/14347\u20101"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Vision is a two\u2010step process, including phototransduction initiated by a retinal\u2010bound opsin photopigment, and subsequent photopigment regeneration wherein the inactive state is restored. Because retinal isomerization, or changing of the molecular structural conformation, drives the activation of opsin photopigments, the reverse process is a necessary part of regeneration. In some animal lineages, a second opsin functions as a photoisomerase that converts retinal back to the isomer needed for the visual opsin, but very little is known about their spectral properties. We investigated spectral variation and spatial expression of scallop retinochrome, an opsin used for light\u2010driven regeneration of molluscan visual photopigments. We show that retinochrome is expressed in scallop retina, along with visual opsins, indicating a conserved role in supporting vision. We compared retinochrome spectral absorption across six species with different ecologies, revealing considerable functional variation possibly associated with photic environments. Finally, we characterized multiple amino acid sites contributing to spectral tuning\u2014shifts in the specific maximally sensitive wavelength of light\u2014of this opsin. These results reveal retinochrome as a functionally dynamic component of the scallop visual cycle that may be adapted to different photic environments across species and highlights spectral tuning in an ocular but otherwise non\u2010visual photopigment, which has been largely overlooked in studying the evolution of visual cycles.<\/jats:p>","DOI":"10.1111\/mec.70390","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T14:56:38Z","timestamp":1780066598000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Widespread Variation in Retinochrome Spectral Absorbance Across Scallop Phylogeny"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9581-2535","authenticated-orcid":false,"given":"Kyle E.","family":"McElroy","sequence":"first","affiliation":[{"name":"Department of Ecology, Evolutionary, and Organismal Biology Iowa State University  Ames Iowa USA"},{"name":"Department of Evolution, Ecology, and Organismal Biology The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0251-346X","authenticated-orcid":false,"given":"Anna K. G.","family":"Ward","sequence":"additional","affiliation":[{"name":"Department of Ecology, Evolutionary, and Organismal Biology Iowa State University  Ames Iowa USA"},{"name":"Department of Evolution, Ecology, and Organismal Biology The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9688-559X","authenticated-orcid":false,"given":"Jorge A.","family":"Audino","sequence":"additional","affiliation":[{"name":"Department of Zoology University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2112-470X","authenticated-orcid":false,"given":"Jeanne M.","family":"Serb","sequence":"additional","affiliation":[{"name":"Department of Ecology, Evolutionary, and Organismal Biology Iowa State University  Ames Iowa USA"},{"name":"Department of Evolution, Ecology, and Organismal Biology The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010vision\u2010100820\u2010094018"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1607989114"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751\u20101097.1994.tb08235.x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1067262"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/evo.12794"},{"key":"e_1_2_11_7_1","first-page":"651","volume-title":"Developments in Aquaculture and Fisheries Science","author":"Brand A. R.","year":"2006"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201010\u2010421"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109258(19)50115\u20106"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.140137"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.s2\u201055.217.49"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.55193"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2016.2743"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2118145119"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/694842"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0257436"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1204609109"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.2298\/ABS180822052G"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.103.6.939"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.10.053"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.165.3890.309"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0952523800174036"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1883"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11152441"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2022.10.014"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/2061331a0"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/214573a0"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0278\u20104327(91)90013\u2010R"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.12.195"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2024.1357873"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx281"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0141390"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0042\u20106989(97)00443\u20104"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.204.19.3333"},{"key":"e_1_2_11_35_1","first-page":"211","volume-title":"Cultivo de Moluscos en Am\u00e9rica Latina. Bogot\u00e1","author":"Illanes J. E.","year":"1990"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00806a020"},{"key":"e_1_2_11_37_1","first-page":"3669","article-title":"An Opsin Homologue in the Retina and Pigment Epithelium","volume":"34","author":"Jiang M.","year":"1993","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.1146"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1086\/694448"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr400107q"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.182923"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.37.22979"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.105527"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10265\u2010016\u20100792\u20105"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.4.1076"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010cellbio\u2010100616\u2010060432"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giy031"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201001927\u20100"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2008986118"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2021.0279"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.38.24583"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915\u2010023\u201001789\u20107"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00359\u2010024\u201001712\u20107"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msad263"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/356433a0"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751\u20101097.1993.tb04956.x"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa015"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2010.07.002"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00359\u2010009\u20100493\u20109"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2026.01.047"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1903559"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature22325"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960\u20109822(02)00417\u20107"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010vision\u2010121820\u2010074736"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006\u20103495(83)84285\u20104"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042\u20106989(86)90083\u20100"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2008211117"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/1011\u20101344(92)80035\u2010T"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12862\u2010016\u20100823\u20109"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2011.1819"},{"key":"e_1_2_11_72_1","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2023"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801288200"},{"key":"e_1_2_11_74_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evw248"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.067140"},{"key":"e_1_2_11_76_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010micro\u2010031721\u2010020452"},{"key":"e_1_2_11_77_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40851\u2010019\u20100150\u20102"},{"key":"e_1_2_11_78_1","first-page":"207","article-title":"On the Evolution of Photoreceptors and Eyes","volume":"10","author":"Salvini\u2010Plawen L. V.","year":"1977","journal-title":"Journal of Evolutionary Biology"},{"key":"e_1_2_11_79_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010025\u201006017\u20109"},{"key":"e_1_2_11_80_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa892"},{"key":"e_1_2_11_81_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja304820p"},{"key":"e_1_2_11_82_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/ict063"},{"key":"e_1_2_11_83_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msad023"},{"key":"e_1_2_11_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.04.061"},{"key":"e_1_2_11_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2019.05.006"},{"key":"e_1_2_11_86_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.242929"},{"key":"e_1_2_11_87_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-031-23216-9_5"},{"key":"e_1_2_11_88_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.048108"},{"key":"e_1_2_11_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042\u20106989(93)90237\u2010Q"},{"key":"e_1_2_11_90_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb\u20102005\u20106\u20103\u2010213"},{"key":"e_1_2_11_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042\u20106989(89)90026\u20106"},{"key":"e_1_2_11_92_1","doi-asserted-by":"publisher","DOI":"10.2108\/zs140094"},{"key":"e_1_2_11_93_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab008"},{"key":"e_1_2_11_94_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.23435"},{"key":"e_1_2_11_95_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12862\u2010021\u201001939\u2010x"},{"key":"e_1_2_11_96_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11243966"},{"key":"e_1_2_11_97_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41559\u2010017\u20100120"},{"key":"e_1_2_11_98_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460\u20102075.1992.tb05261.x"},{"key":"e_1_2_11_99_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt447"},{"key":"e_1_2_11_100_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks966"},{"key":"e_1_2_11_101_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004884"},{"key":"e_1_2_11_102_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0109-x"},{"key":"e_1_2_11_103_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2015.06.007"},{"key":"e_1_2_11_104_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010020\u20102683\u20100"}],"container-title":["Molecular Ecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mec.70390","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mec.70390","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mec.70390","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mec.70390","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T01:11:28Z","timestamp":1781572288000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mec.70390"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,29]]},"references-count":103,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/mec.70390"],"URL":"https:\/\/doi.org\/10.1111\/mec.70390","archive":["Portico"],"relation":{},"ISSN":["0962-1083","1365-294X"],"issn-type":[{"value":"0962-1083","type":"print"},{"value":"1365-294X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,29]]},"assertion":[{"value":"2025-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70390"}}