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Sloan Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000879","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2219279"],"award-info":[{"award-number":["2219279"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1942250"],"award-info":[{"award-number":["1942250"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Heat stress can disrupt coordinated regulatory processes underpinning normal development, resulting in embryonic malformation and inviability. Embryonic buffering against thermal challenge may therefore play a crucial role in individual survival and population resilience under heat stress. Yet, the molecular mechanisms underpinning embryonic defence against ecologically\u2010meaningful heat stresses remain poorly understood. We investigated the regulatory responses of brown anole (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Anolis sagrei<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) embryos to three patterns of heat stress we find will increase under near\u2010future climate warming. Across chronic, persistent, and acute heat treatments, embryos exhibited rapid transcriptome\u2010wide disruption followed by recovery toward normal developmental trajectories within 24\u2009h. Despite this broad perturbation, a core regulatory module governing forebrain and craniofacial development remained strongly preserved across heat insults. Heat stress induced widespread transcriptional suppression alongside increased reliance of post\u2010transcriptional regulation and a dose\u2010dependent slowing of RNA flux, consistent with mitigation of proteotoxic stress. Concurrently, embryos rewired regulatory networks through recruitment of extramodular stress\u2010response genes and strengthening co\u2010expression within developmental pathways. Together, these results reveal a hierarchical regulatory strategy in which embryos buffer essential developmental programs while flexibly reconfiguring peripheral networks to manage cellular stress. This systems\u2010level resilience underscores the importance of early\u2010life adaptive regulatory plasticity in shaping organismal responses to climate warming.\n                  <\/jats:p>","DOI":"10.1111\/mec.70456","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T23:14:20Z","timestamp":1783725260000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Regulatory Responses of Ectothermic Embryos to Predicted Heat Stresses Under Near\u2010Future Climate Change"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3584-5399","authenticated-orcid":false,"given":"Reena H.","family":"Walker","sequence":"first","affiliation":[{"name":"Department of Ecology &amp; Evolutionary Biology Princeton University  Princeton New Jersey 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