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Plastic changes in adult animals in preparation for migration are well known, but the differences in early development between migratory and non\u2010migratory forms are not well documented. These differences might contribute to reproductive isolation because of the potentially substantial divergence in metabolism and physiology. Here we document substantial differences between migratory ecotypes in sarcomeric development and energetic pathways from immediately after hatching. On North Uist (Scottish Western Isles), migratory and non\u2010migratory (\u2018resident\u2019) three\u2010spined stickleback (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Gasterosteus aculeatus<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) ecotypes breed in sympatry in brackish lagoons, providing an ideal system for investigating early developmental and energetic strategies associated with migration. Using relative\u2010quantitative proteomics, we compared protein abundance in migratory, resident and reciprocal hybrid crosses of the three\u2010spined stickleback 2\u2009days after hatching. Migratory stickleback exhibited higher abundance of muscle, metabolic and collagen\u2010associated proteins, indicating early investment in growth and preparation for the energetic demands of migration and sustained swimming. In contrast, resident stickleback showed increased abundance of maternal provisioning proteins, delayed hatching and higher hatching success, consistent with a strategy of producing fewer but high\u2010quality offspring. Hybrids generally exhibited intermediate protein abundances, but there was some evidence of negative transgression in proteins related to mitochondrial function which might contribute to reduced fitness through mitonuclear incompatibility. Together, these results reveal early biochemical differences consistent with divergent energy allocation and reproductive strategies between migratory and resident stickleback.\n                  <\/jats:p>","DOI":"10.1111\/mec.70494","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T13:57:41Z","timestamp":1786975061000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Proteomic Differences Reveal Early Development of Contrasting Energetic Strategies Between Divergent Migratory Ecotypes in Three\u2010Spined Stickleback"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4399-4645","authenticated-orcid":false,"given":"Megan","family":"Barnes","sequence":"first","affiliation":[{"name":"School of Life Sciences University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Dowle","sequence":"additional","affiliation":[{"name":"Department of Biology, Bioscience Technology Facility University of York  York UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2102-6130","authenticated-orcid":false,"given":"Andrew D. 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