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However, the genetic consequences of these life\u2010cycle changes remain unclear.\n                    <jats:italic>Rhinogobius<\/jats:italic>\n                    is a speciose genus of freshwater gobies widely distributed in Asia, including non\u2010migratory\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. duospilus<\/jats:italic>\n                    <\/jats:styled-content>\n                    and facultatively amphidromous\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. similis<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Using genome\u2010wide SNPs from Hong Kong, southern China, we compared the genetic diversity and structure of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. duospilus<\/jats:italic>\n                    <\/jats:styled-content>\n                    (six populations), non\u2010landlocked\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. similis<\/jats:italic>\n                    <\/jats:styled-content>\n                    (three populations), and landlocked\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. similis<\/jats:italic>\n                    <\/jats:styled-content>\n                    (four populations) that had been isolated for 58\u2013106\u2009years. We also used demographic modelling to simulate patterns of gene flow of a panmictic amphidromous population of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. similis<\/jats:italic>\n                    <\/jats:styled-content>\n                    fragmented by dam construction 60\u2009years ago.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Rhinogobius duospilus<\/jats:italic>\n                    <\/jats:styled-content>\n                    populations were deeply divergent, with low genetic diversity; interpopulation differentiation reflected the underlying paleodrainage architecture. In contrast, non\u2010landlocked\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. similis<\/jats:italic>\n                    <\/jats:styled-content>\n                    populations were genetically homogenous with high genetic diversity. However, all four landlocked populations of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. similis<\/jats:italic>\n                    <\/jats:styled-content>\n                    were highly differentiated, with three populations above tall (&gt;\u200915\u2009m) dams exhibiting comparable genetic clustering to non\u2010migratory\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. duospilus<\/jats:italic>\n                    <\/jats:styled-content>\n                    , although overall genetic diversity remained high. Demographic modelling of a landlocked\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. similis<\/jats:italic>\n                    <\/jats:styled-content>\n                    population indicated negligible migration into the reservoir and none outward, in accordance with the high levels of genetic divergence among landlocked populations. The effective population size of the original amphidromous population was small (Ne\u2009=\u200981), but the population landlocked above the reservoir for 60\u2009years grew eightfold (Ne\u2009=\u2009679). Our modelling suggests that even a limited level of immigration was sufficient to minimize genetic drift and lessen interpopulation divergence.\n                  <\/jats:p>","DOI":"10.1111\/mec.70534","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T11:00:49Z","timestamp":1787914849000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Contrasting Patterns of Genetic Isolation Among Diadromous, Landlocked, and Non\u2010Migratory Gobiid Fish Populations"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0412-9429","authenticated-orcid":false,"given":"Jeffery C. 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