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Through the Citizens of the Sea initiative, we deployed standardized environmental DNA (eDNA) sampling kits on 27 sailing vessels during the 2024 Pacific Rally circumnavigating between New Zealand, Tonga, and Fiji, generating 728 surface ocean eDNA samples across 1.5\u2009million km\n                    <jats:sup>2<\/jats:sup>\n                    . Using three complementary metabarcoding markers (bacterial 16S rRNA, eukaryotic 18S rRNA and metazoan Cytochrome Oxidase I genes), and following rigorous bioinformatic quality control, we obtained 31,177 unique Amplicon Sequence Variants (ASVs), providing the first high\u2010resolution plankton biodiversity assessment of this understudied region. After accounting for human\u2010derived variation, all metabarcoding markers revealed clear latitudinal patterns in alpha diversity and community composition. ASV richness increased significantly with sea\u2010surface temperature and community turnover was strongly correlated with gradients in temperature and chlorophyll\u2010a. Comparisons between eDNA\u2010inferred phytoplankton functional groups and satellite\u2010based estimates showed moderate agreement for prokaryote\u2010dominated communities (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.27) but a weak correlation for eukaryotic phytoplankton (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009&lt;\u20090.15), reflecting functional\u2010type\u2010specific mismatches between molecular and remote sensing approaches. Our results reinforce that citizen science fleets can generate high\u2010quality oceanographic eDNA datasets at scales unattainable by research expeditions, while demonstrating the strong potential of these initiatives to bridge molecular observations with satellite\u2010derived assessments and improve our understanding of open ocean plankton biodiversity.\n                  <\/jats:p>","DOI":"10.1111\/mec.70571","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:18:21Z","timestamp":1790752701000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Citizen Sailors Provide a Detailed Map of Open\u2010Ocean Surface Biodiversity and Help Link\n                    <scp>eDNA<\/scp>\n                    Data to Satellite 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