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Microbial communities may prevent pollen provision spoilage, liberate nutrients from pollen, supply direct nutrition and\/or suppress pathogens, though these functions appear context\u2010dependent and variable among hosts. To determine whether bee evolutionary history and ecological traits structure pollen provision microbiomes, we analyzed 345 \u2018live\u2019 brood cell pollen provisions representing 39 species, 18 tribes and six bee families spanning diverse life histories, diet breadths, floral resource use and nesting substrates. Microbial composition was determined by amplicon sequencing of bacterial 16S rRNA (V5\u2013V7) and fungal ITS (1 and 2) regions. Bacterial richness and community composition were strongly structured by bee taxonomy and were also associated with several ecological traits, while community composition was significantly associated with host phylogeny. Fungal richness also varied among bee taxa and ecological groups, whereas fungal community composition showed greater variability and weaker, though detectable, taxonomic structure, with comparatively small ecological\u2010trait effects. Across phylogenetically distant bee tribes, pollen provisions were often dominated by lactic\u2010 or acetic acid\u2010producing bacteria, indicating a strategy of microbial fermentation that reduces bacterial richness and may enhance food preservation. Together, these results reveal broad evolutionary and ecological patterns underlying the microbiome of solitary bee brood cells and suggest that acid\u2010producing bacteria play a stabilizing role in the preservation and function of pollen provisions.<\/jats:p>","DOI":"10.1111\/mec.70578","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T15:39:31Z","timestamp":1791992371000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Acidogenic Bacteria Dominate Brood Pollen Provisions Across Diverse Bee Taxa"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9521-6282","authenticated-orcid":false,"given":"Jordan G.","family":"Kueneman","sequence":"first","affiliation":[{"name":"Department of Entomology Cornell University  Ithaca New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda","family":"Argueta\u2010Guzm\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental Sciences University of California Merced  Merced California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quinn S.","family":"McFrederick","sequence":"additional","affiliation":[{"name":"Department of Entomology University of California Riverside  Riverside California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel L.","family":"Vannette","sequence":"additional","affiliation":[{"name":"Department of Entomology and Nematology University of California Davis  Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn A.","family":"Steffan","sequence":"additional","affiliation":[{"name":"Department of Entomology University of Wisconsin  Madison Wisconsin USA"},{"name":"US Department of Agriculture Agricultural Research Service  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen L.","family":"Buchmann","sequence":"additional","affiliation":[{"name":"Department of Entomology University of Arizona  Tucson Arizona USA"},{"name":"Department of Ecology and Evolutionary Biology University of Arizona  Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Roubik","sequence":"additional","affiliation":[{"name":"Smithsonian Tropical Research Institute  Panama City Panama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William T.","family":"Wcislo","sequence":"additional","affiliation":[{"name":"Smithsonian Tropical Research Institute  Panama City Panama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan N.","family":"Danforth","sequence":"additional","affiliation":[{"name":"Department of Entomology Cornell University  Ithaca New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2020.08.006"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2023.07.005"},{"key":"e_1_2_12_4_1","first-page":"1","volume-title":"Wiley StatsRef: Statistics Reference Online","author":"Anderson M. 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