{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T07:40:20Z","timestamp":1792222820189,"version":"4.4.0"},"reference-count":76,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2020\u2010118027GB\u2010I00"],"award-info":[{"award-number":["PID2020\u2010118027GB\u2010I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PRE2018\u2010084009"],"award-info":[{"award-number":["PRE2018\u2010084009"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","award":["AICO\/2021\/113"],"award-info":[{"award-number":["AICO\/2021\/113"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Seminal fluid proteins (SFPs) play crucial roles in reproduction by shaping female post\u2010mating physiology and behaviour, male sperm competition and sexual conflict between the sexes. However, we know little about how abiotic ecological factors regulate SFP expression and transfer. Here, we use quantitative proteomics in adult flies from a wild\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    population to test how exposure to natural temperature variation (20\u00b0C, 24\u00b0C and 28\u00b0C) across two time scales (48\u2009h and 13d), and under either no\u2010 or high\u2010sperm competition risk, interact to shape SFP production and transfer. Short\u2010term exposure to different temperatures resulted in lower SFP production and transfer at 24\u00b0C under no sperm competition risk, but no differences under high sperm competition risk. In contrast, both the production and transfer of SFPs were consistently reduced across sperm competition treatments after long\u2010term (13d) exposure to low (20\u00b0C) and high (28\u00b0C) temperatures, including for key proteins in the sex peptide and ovulin networks that mediate female post\u2010copulatory responses, sperm competition and sexual conflict. Our results show that natural temperature fluctuations can have a strong effect on SFP production and transfer, and thus on post\u2010copulatory sexual selection dynamics.\n                  <\/jats:p>","DOI":"10.1111\/mec.70599","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T06:46:52Z","timestamp":1792219612000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chilling Out or Heating Up? Thermal Plasticity of Seminal Fluid Proteins in\n                    <i>Drosophila melanogaster<\/i>"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7408-7327","authenticated-orcid":false,"given":"Claudia","family":"Londo\u00f1o\u2010Nieto","sequence":"first","affiliation":[{"name":"Ethology Lab, Cavanilles Institute of Biodiversity and Evolutionary Biology University of Valencia  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Garc\u00eda\u2010Roa","sequence":"additional","affiliation":[{"name":"Ethology Lab, Cavanilles Institute of Biodiversity and Evolutionary Biology University of Valencia  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2260-2948","authenticated-orcid":false,"given":"Stuart","family":"Wigby","sequence":"additional","affiliation":[{"name":"Department of Evolution, Ecology and Behaviour Institute of Infection, Veterinary &amp; Ecological Sciences, University of Liverpool  Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3228-5480","authenticated-orcid":false,"given":"Irem","family":"Sepil","sequence":"additional","affiliation":[{"name":"Department of Biology University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1525-6522","authenticated-orcid":false,"given":"Pau","family":"Carazo","sequence":"additional","affiliation":[{"name":"Ethology Lab, Cavanilles Institute of Biodiversity and Evolutionary Biology University of Valencia  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"crossref","DOI":"10.1515\/9781400850600","volume-title":"Sexual Conflict","author":"Arnqvist G.","year":"2005"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010ento\u2010120709\u2010144823"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ele.14355"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2020.3094"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004775"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom13020378"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v080.i01"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.9563"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.T400006\u2010MCP200"},{"key":"e_1_2_12_11_1","first-page":"511","article-title":"Seminal Fluid\u2010Mediated Fitness Traits in Drosophila","volume":"87","author":"Chapman T.","year":"2001","journal-title":"Genetics"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1631635100"},{"key":"e_1_2_12_13_1","first-page":"168","article-title":"A Simple Method to Achieve Consistent Larval Density in Bottle Cultures","volume":"84","author":"Clancy D. 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Series B, Biological Sciences"},{"issue":"4","key":"e_1_2_12_72_1","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.cub.2005.01.051","article-title":"Sex Peptide Causes Mating Costs in Female \n                     Drosophila melanogaster","volume":"15","author":"Wigby S.","year":"2005","journal-title":"Current Biology"},{"key":"e_1_2_12_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/1365\u20102435.12515"},{"key":"e_1_2_12_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2009.03.036"},{"key":"e_1_2_12_75_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2017.0424"},{"key":"e_1_2_12_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201046332\u20106"},{"key":"e_1_2_12_77_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467\u20109868.2005.00503.x"}],"container-title":["Molecular Ecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mec.70599","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mec.70599","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mec.70599","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T06:46:58Z","timestamp":1792219618000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mec.70599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":76,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/mec.70599"],"URL":"https:\/\/doi.org\/10.1111\/mec.70599","archive":["Portico"],"relation":{},"ISSN":["0962-1083","1365-294X"],"issn-type":[{"value":"0962-1083","type":"print"},{"value":"1365-294X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-05-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70599"}}