{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T21:16:03Z","timestamp":1771362963250,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,1,20]],"date-time":"2018-01-20T00:00:00Z","timestamp":1516406400000},"content-version":"am","delay-in-days":203,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2017,7,10]],"date-time":"2017-07-10T00:00:00Z","timestamp":1499644800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1150235"],"award-info":[{"award-number":["1150235"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1538161"],"award-info":[{"award-number":["1538161"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5U01\u2010HL117721"],"award-info":[{"award-number":["5U01\u2010HL117721"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U54HL112309"],"award-info":[{"award-number":["U54HL112309"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL121264"],"award-info":[{"award-number":["R01HL121264"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Vaso\u2010occlusive crisis (VOC) is a complex process that occurs in patients with sickle cell disease (SCD) and is often associated with pain and urgent hospitalization. A major instigator of VOC is microvascular obstruction by pathologically stiffened sickle red blood cells (RBCs), and thus, therapy relies heavily on optimizing intravenous fluid (IVF) hydration to increase RBC deformability. However, no evidence\u2010based guidelines regarding the choice of IVF currently exist. We therefore analyzed alterations in biomechanical properties of sickle RBCs isolated from patients with homozygous SCD (hemoglobin SS) after exposure to different osmolarities of clinical IVF formulations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Atomic force microscopy (AFM) was used to assess stiffness of RBCs after exposure to different IVFs. A microfluidic model of the human capillary system was used to assess transit time (TT) and propensity to occlusion after exposure to the different IVF formulations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sickle RBCs exposed to normal saline (NS) had increased stiffness, TTs, and propensity to microchannel occlusion compared to other osmolarities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>NS, an IVF formulation often used to treat patients with SCD during VOC, may induce localized microvascular obstruction due to alterations of sickle RBC biomechanical properties.<\/jats:p><\/jats:sec>","DOI":"10.1111\/micc.12353","type":"journal-article","created":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T18:34:19Z","timestamp":1484937259000},"source":"Crossref","is-referenced-by-count":23,"title":["Normal saline is associated with increased sickle red cell stiffness and prolonged transit times in a microfluidic model of the capillary system"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3860-3619","authenticated-orcid":false,"given":"Marcus A.","family":"Carden","sequence":"first","affiliation":[{"name":"Division of Pediatric Hematology\/Oncology Department of Pediatrics Aflac Cancer Center and Blood Disorders Center of Children's Healthcare of Atlanta Emory University School of Medicine  Atlanta GA USA"},{"name":"The Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology Emory University  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meredith","family":"Fay","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology\/Oncology Department of Pediatrics Aflac Cancer Center and Blood Disorders Center of Children's Healthcare of Atlanta Emory University School of Medicine  Atlanta GA USA"},{"name":"The Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology Emory University  Atlanta GA USA"},{"name":"Parker H. Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology  Atlanta GA USA"},{"name":"Institute for Electronics and Nanotechnology Georgia Institute of Technology  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Sakurai","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology\/Oncology Department of Pediatrics Aflac Cancer Center and Blood Disorders Center of Children's Healthcare of Atlanta Emory University School of Medicine  Atlanta GA USA"},{"name":"The Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology Emory University  Atlanta GA USA"},{"name":"Parker H. Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology  Atlanta GA USA"},{"name":"Institute for Electronics and Nanotechnology Georgia Institute of Technology  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brynn","family":"McFarland","sequence":"additional","affiliation":[{"name":"Georgia Tech School of Chemical &amp; Biomolecular Engineering  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sydney","family":"Blanche","sequence":"additional","affiliation":[{"name":"The Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology Emory University  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caleb","family":"DiPrete","sequence":"additional","affiliation":[{"name":"The Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology Emory University  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clinton H.","family":"Joiner","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology\/Oncology Department of Pediatrics Aflac Cancer Center and Blood Disorders Center of Children's Healthcare of Atlanta Emory University School of Medicine  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todd","family":"Sulchek","sequence":"additional","affiliation":[{"name":"Parker H. Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology  Atlanta GA USA"},{"name":"George W. Woodruff School of Mechanical Engineering Georgia Institute of Technology  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilbur A.","family":"Lam","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology\/Oncology Department of Pediatrics Aflac Cancer Center and Blood Disorders Center of Children's Healthcare of Atlanta Emory University School of Medicine  Atlanta GA USA"},{"name":"The Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology Emory University  Atlanta GA USA"},{"name":"Parker H. Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology  Atlanta GA USA"},{"name":"Institute for Electronics and Nanotechnology Georgia Institute of Technology  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/10739680490278277"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-070909-105339"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199107043250103"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2015.8"},{"issue":"3","key":"e_1_2_9_6_1","first-page":"Cd005406","article-title":"Fluid replacement therapy for acute episodes of pain in people with sickle cell disease","author":"Okomo U","year":"2015","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V77.2.214.214"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00052.2003"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199709113371107"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V70.5.1245.1245"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V58.6.1057.1057"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10739680490278312"},{"key":"e_1_2_9_13_1","unstructured":"2016 UpToDate Waltham MA MR DeBaun TW Post Vasoocclusive pain management in sickle cell disease"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1998.tb00989.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.4.1130.003k03a_1130_1136"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046609"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-08-043570"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1508920113"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2016.09.005"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13118"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2015.01.010"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI58753"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/b802931h"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707122105"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3002738"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2010.11.008"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/0309324711398809"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-197310000-00005"}],"container-title":["Microcirculation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmicc.12353","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/micc.12353","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/micc.12353","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1111\/micc.12353","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/micc.12353","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T06:05:39Z","timestamp":1694498739000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/micc.12353"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1111\/micc.12353"],"URL":"https:\/\/doi.org\/10.1111\/micc.12353","archive":["Portico"],"relation":{},"ISSN":["1073-9688","1549-8719"],"issn-type":[{"value":"1073-9688","type":"print"},{"value":"1549-8719","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7]]},"article-number":"e12353"}}