{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T21:39:52Z","timestamp":1773697192151,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,3,31]],"date-time":"2018-03-31T00:00:00Z","timestamp":1522454400000},"content-version":"am","delay-in-days":242,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2017,8,3]],"date-time":"2017-08-03T00:00:00Z","timestamp":1501718400000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["LP110200543"],"award-info":[{"award-number":["LP110200543"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001782","name":"University of Melbourne","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001782","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Short\u2010term IV sRLX (recombinant human relaxin\u20102) infusion enhances endothelium\u2010dependent relaxation in mesenteric arteries. This is initially underpinned by increased<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>followed by a transition to prostacyclin. The effects of short\u2010term IV sRLX treatment on pressure\u2010induced myogenic tone and vascular remodeling in these arteries are unknown. Therefore, we investigated the effects of sRLX infusion on pressure\u2010induced myogenic tone and passive mechanical wall properties in mesenteric arteries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mesenteric artery myogenic tone and passive mechanics were examined after 48\u2010hours and 10\u2010days infusion of sRLX. Potential mechanisms of action were assessed by pressure myography,<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>, and Western blot analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Neither 48\u2010hours nor 10\u2010days sRLX treatment had significant effects on myogenic tone, passive arterial wall stiffness, volume compliance, or axial lengthening. However, in 48\u2010hours sRLX \u2010treated rats, incubation with the<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>synthase blocker L\u2010<jats:styled-content style=\"fixed-case\">NAME<\/jats:styled-content>significantly increased myogenic tone (<jats:italic>P<\/jats:italic>&lt;.05 vs placebo), demonstrating an increased contribution of<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>to the regulation of myogenic tone.<jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content>dimerization, but not phosphorylation, was significantly upregulated in the arteries of sRLX \u2010treated rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In mesenteric arteries, 48\u2010hours sRLX treatment upregulates the role of<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>in the regulation of myogenic tone by enhancing<jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content>dimerization, without altering overall myogenic tone or vascular remodeling.<\/jats:p><\/jats:sec>","DOI":"10.1111\/micc.12371","type":"journal-article","created":{"date-parts":[[2017,4,3]],"date-time":"2017-04-03T20:58:51Z","timestamp":1491253131000},"source":"Crossref","is-referenced-by-count":10,"title":["Short\u2010term (48\u00a0hours) intravenous serelaxin infusion has no effect on myogenic tone or vascular remodeling in rat mesenteric arteries"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9773-4972","authenticated-orcid":false,"given":"Maria","family":"Jelinic","sequence":"first","affiliation":[{"name":"School of BioSciences The University of Melbourne Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen Huei","family":"Leo","sequence":"additional","affiliation":[{"name":"School of BioSciences The University of Melbourne Parkville Victoria 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